Microsatellite DNA markers for Plasmopara viticola, the causal agent of downy mildew of grapes
Microsatellite DNA markers for Plasmopara viticola, the causal agent of downy mildew of grapes
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DOI：
10.1111/j.1471-8286.2005.01240.x
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发表时间：
2006-06-01
期刊：
影响因子：
--
通讯作者：
Corio-Costet, M. F.
中科院分区：
文献类型：
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作者：
Delmotte, F.;Chen, W. J.;Corio-Costet, M. F.
微卫星位点是从葡萄生单轴霉（卵菌纲）中分离出来的，葡萄霜霉病是世界范围内葡萄树最具破坏性的真菌病害之一。从富集的部分基因组文库中获得了七个多态性位点。所有位点均观察到较低的遗传多样性，观察到的平均等位基因数为 3.75，观察到的杂合度范围为 0.074 至 0.547。对三个密切相关的类群的交叉扩增测试表明，两个基因座可用于其他卵菌物种。这些微卫星位点被证明可用于群体遗传分析。
Microsatellite loci were isolated from Plasmopara viticola (Oomycetes), the causal agent of downy mildew of grape, one of the most damaging fungal diseases of grapevine worldwide. Seven polymorphic loci were obtained from an enriched partial genomic library. A low genetic diversity was observed at all loci, with a mean observed allele number of 3.75 and an observed heterozygosity ranging from 0.074 to 0.547. Cross-amplification tests on three closely related taxa indicated that two loci could be used in other Oomycetes species. These microsatellite loci were proved to be useful for population genetic analysis.