Design and Fabrication of a Flexible Opto-Electric Biointerface for Multimodal Optical Fluorescence and Electrical Recording
Design and Fabrication of a Flexible Opto-Electric Biointerface for Multimodal Optical Fluorescence and Electrical Recording
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DOI：
10.1021/acsaelm.2c01732
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发表时间：
2023-02-27
影响因子：
4.7
通讯作者：
Lu，Luyao
中科院分区：
文献类型：
--
作者：
Obaid，Sofian N.;Quirion，Nathaniel;Lu，Luyao
光学荧光和细胞活动的电监测是研究器官功能的两种有力方法。同时记录光学和电子数据类型将提供互补的信息，并利用每种方法的优势。然而，能够同时记录来自微电极下相同细胞群的光信号的设备尚未被广泛探索，并且仍然是一个巨大的技术挑战。这项工作提出了一种创新的柔性光电器件，该器件将透明金纳米网格微电极单片集成在微尺度发光二极管，光电探测器和光学滤波器之上，以实现共定位无串扰光学荧光和电记录。优化后的金纳米网格微电极具有优异的光学透明度（>81%）和低归一化1 kHz电化学阻抗（6.3 Ω cm2）。光学记录子系统提供高波长选择性（> 1300）和线性度（R2> 0.99），用于激发和捕获与体内应用相关的各种荧光报告的测量范围内的绿色荧光，并且具有最小的热效应。该光电器件在40天的浸泡和5000次的重复机械弯曲下具有显著的耐久性。这项工作可能为构建具有无串扰光学和电模态的机械柔性生物界面提供了一种通用方法，在基础和临床研究中具有广泛的应用潜力。
Optical fluorescence and electrical monitoring of cell activity are two powerful approaches to study organ functions. Simultaneous recording of optical and electrical data types will provide complementary information from and take advantage of each approach. However, devices that can concurrently record optical signals from the same cell population underneath the microelectrodes have not been widely explored and remain a grand technical challenge. This work presents an innovative flexible opto-electric device that monolithically integrates transparent gold nanogrid microelectrodes directly above microscale light-emitting diodes, photodetectors, and optical filters to achieve co-localized crosstalk-free optical fluorescence and electrical recording. The optimized gold nanogrid microelectrodes show an excellent optical transparency (>81%) and a low normalized 1 kHz electrochemical impedance (6.3 Ω cm2). The optical recording subsystem offers high wavelength selectivity (>1,300) and linearity (R2> 0.99) for exciting and capturing green fluorescence from various fluorescent reporters in measurement ranges relevant for in vivo applications with minimal thermal effects. The opto-electric device exhibits remarkable durability under soaking for 40 days and repetitive mechanical bending for 5000 cycles. The work may provide a versatile approach for constructing mechanically compliant biointerfaces containing crosstalk-free optical and electrical modalities with widespread application potentials in basic and clinical research.