Interferon-induced guanylate binding protein-1 (GBP-1) mediates an antiviral effect against vesicular stomatitis virus and encephalomyocarditis virus
Interferon-induced guanylate binding protein-1 (GBP-1) mediates an antiviral effect against vesicular stomatitis virus and encephalomyocarditis virus
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DOI：
10.1006/viro.1999.9614
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发表时间：
1999-03-30
期刊：
影响因子：
3.7
通讯作者：
Rubin, BY
中科院分区：
文献类型：
--
作者：
Anderson, SL;Carton, JM;Rubin, BY
利用组成型表达载体在HeLa细胞中表达了人鸟苷酸结合蛋白-1 （hGBP-1）的cDNA。稳定转染的表达hGBP-1的克隆对水疱性口炎病毒（VSV）和脑心肌炎病毒（EMCV）介导的细胞病变作用表现出抗性，并且在感染这些病毒后产生的病毒子代比对照细胞少。为了研究hGBP-1在ifn介导的抗病毒作用中所起的作用，我们用表达hGBP-1反义RNA的构建体稳定转染细胞。经ifn - α处理的表达反义rna的细胞感染VSV产生的病毒量与亲本细胞系相当，而经ifn - α处理的转染细胞感染VSV和经ifn - γ处理的转染细胞感染EMCV产生的病毒量远远超过亲本细胞系。这些结果表明，GBP-1介导对VSV和EMCV的抗病毒作用，并在ifn介导的对这些病毒的抗病毒反应中发挥作用，(C) 1999学术出版社。
A cDNA encoding the human guanylate binding protein-1 (hGBP-1) was expressed in HeLa cells using a constitutive expression vector. Stably transfected clones expressing hGBP-1 exhibited resistance to the cytopathic effect mediated by both vesicular stomatitis virus (VSV) and encephalomyocarditis virus (EMCV) and produced less viral progeny than control cells following infection with these viruses. To study the role hGBP-1 plays in the IFN-mediated antiviral effect, cells were stably transfected with a construct expressing antisense RNA for hGBP-1. VSV infection of IFN-alpha-treated antisense RNA-expressing cells produced an amount of virus comparable to that produced in the parental cell line, while EMCV infection of the IFN-alpha-treated transfected cells and VSV and EMCV infection of the IFN-gamma-treated transfected cells produced far more virus than was produced in the parental cell line. These results demonstrate that GBP-1 mediates an antiviral effect against VSV and EMCV and plays a role in the IFN-mediated antiviral response against these viruses, (C) 1999 Academic Press.