Self-formed adaptor PCR: a simple and efficient method for chromosome walking
Self-formed adaptor PCR: a simple and efficient method for chromosome walking
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DOI：
10.1128/aem.02973-06
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发表时间：
2007-08-01
影响因子：
4.4
通讯作者：
Li, Shunpeng
中科院分区：
文献类型：
--
作者：
Wang, Shiming;He, Jian;Li, Shunpeng
我们开发了一种可用于染色体行走的自形成接头PCR（称为SEFA PCR）。扩增的侧翼序列大部分长度大于2.0 kb，有的长度甚至达到6.0 kb。SEFA PCR方法简单、高效，在复杂基因组中未知序列的分离中具有广泛的应用前景。
We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequences in complex genomes.