Direct enantiomeric resolution of cyclic alcohol derivatives of polycyclic aromatic hydrocarbons by chiral stationary phase high-performance liquid chromatography.
Direct enantiomeric resolution of cyclic alcohol derivatives of polycyclic aromatic hydrocarbons by chiral stationary phase high-performance liquid chromatography.
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DOI：
10.1016/s0021-9673(00)95028-3
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Shen K. Yang;Xiao-Ching Li
中科院分区：
文献类型：
--
作者：
Shen K. Yang;Xiao-Ching Li
用高效液相色谱法分离了菲、苯并[a]蒽、苯并[a]芘、胆蒽和3-甲基胆蒽等环醇衍生物的对映体。通过紫外-可见吸收光谱、质谱和圆二色谱分析，对对映体进行了拆分。该方法已用于测定胆蒽和3-甲基胆蒽在大鼠肝微粒体中代谢形成的1-羟基胆蒽和1-羟基-3-甲基胆蒽的光学纯度。
The enantiomers of some cyclic alcohol derivatives of phenanthrene, benz-[a]anthracene, benzo[a]pyrene, cholanthrene, and 3-methylcholanthrene were resolved by high-performance liquid chromatography using a commercially available preparative column packed with an (R)-N-(3,5-dinitrobenzoyl)phenylglycine ionically bonded to γ-aminopropylsilanized silica. Resolution of enantiomers was confirmed by ultraviolet—visible absorption, mass and circular dichroism spectral analyses. This method has been applied to the determination of optical purity of 1-hydroxycholanthrene and 1-hydroxy-3-methylcholanthrene formed in the metabolism of cholanthrene and 3-methylcholanthrene, respectively, by rat liver microsomes.