Selective ex-vivo photothermal ablation of human pancreatic cancer with albumin functionalized multiwalled carbon nanotubes
Selective ex-vivo photothermal ablation of human pancreatic cancer with albumin functionalized multiwalled carbon nanotubes
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DOI：
10.2147/ijn.s19013
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发表时间：
2011-01-01
影响因子：
8
通讯作者：
Iancu, Cornel
中科院分区：
文献类型：
--
作者：
Mocan, Lucian;Tabaran, Flaviu A.;Iancu, Cornel
用生物功能化碳纳米管标记的癌细胞的激光介导消融的过程通常被称为“纳米光热疗法”。我们在此提出了一种方法，选择性nanophotothermolisys胰腺癌（PC）使用多壁碳纳米管（MWCNTs）功能化与人血清白蛋白（HSA）。为了测试这些纳米生物缀合物的治疗价值，我们开发了一个离体实验平台。手术切除标本PC患者保存在冷介质中，并通过动脉内灌注保持活力。此外，HSA-MWCNT已经在超声引导下在胰腺大动脉中动脉内施用。共聚焦和透射电子显微镜结合免疫组织化学染色证实了人前列腺组织内HSA-MWCNTs的选择性积累。在动脉内施用HSA-MWCNT后，样本的外部激光照射显著地产生了恶性组织的广泛坏死，而对周围健康实质没有任何有害影响。我们已经获得了选择性光热消融的恶性组织的基础上选择性内化的多壁碳纳米管与HSA货物内的胰腺癌后，离体动脉内灌注。
The process of laser-mediated ablation of cancer cells marked with biofunctionalized carbon nanotubes is frequently called "nanophotothermolysis". We herein present a method of selective nanophotothermolisys of pancreatic cancer (PC) using multiwalled carbon nanotubes (MWCNTs) functionalized with human serum albumin (HSA). With the purpose of testing the therapeutic value of these nanobioconjugates, we have developed an ex-vivo experimental platform. Surgically resected specimens from patients with PC were preserved in a cold medium and kept alive via intra-arterial perfusion. Additionally, the HSA-MWCNTs have been intra-arterially administered in the greater pancreatic artery under ultrasound guidance. Confocal and transmission electron microscopy combined with immunohistochemical staining have confirmed the selective accumulation of HSA-MWCNTs inside the human PC tissue. The external laser irradiation of the specimen has significantly produced extensive necrosis of the malign tissue after the intra-arterial administration of HSA-MWCNTs, without any harmful effects on the surrounding healthy parenchyma. We have obtained a selective photothermal ablation of the malign tissue based on the selective internalization of MWCNTs with HSA cargo inside the pancreatic adenocarcinoma after the ex-vivo intra-arterial perfusion.