Circadian clock genes link photoperiodic signals to lipid accumulation during diapause preparation in the diapause-destined female cabbage beetles Colaphellus bowringi.
Circadian clock genes link photoperiodic signals to lipid accumulation during diapause preparation in the diapause-destined female cabbage beetles Colaphellus bowringi.
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DOI：
10.1016/j.ibmb.2018.11.001
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发表时间：
2019
影响因子：
3.8
通讯作者：
Li Zhu;Zhong Tian;Shuang Guo;Wen Liu;F. Zhu;Xiaoping Wang
中科院分区：
文献类型：
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作者：
Li Zhu;Zhong Tian;Shuang Guo;Wen Liu;F. Zhu;Xiaoping Wang
许多生物进化出一系列的适应，如昆虫的休眠或滞育，使它们能够承受季节性的不利条件。昆虫在滞育诱导期接收到的光周期信号对滞育的发生具有不可逆的影响。昆虫在进入滞育之前的滞育准备期间，在滞育诱导阶段之后继续暴露于滞育诱导光周期。然而，在滞育准备阶段（DPP）期间经历的光周期信号如何调节滞育仍然很不清楚。在本文中，我们调查了这在菜青虫。白菜甲具有长日照诱导的兼性生殖滞育和不同的滞育诱导期和滞育准备期，是研究光周期信号对DPP影响的理想实验模型。我们发现，女性白菜甲虫的脂质含量下降后滞育注定（DD）个人暴露于滞育抑制光周期期间DPP。昼夜节律负调控基因perandtim可能参与了甲虫在DPP期间的光周期反应，在DD雌性中，perandtim在DPP期间表现出明显的昼夜节律振荡和光周期反应，在DD雌性中，这些基因的敲低导致其脂质含量降低，perandtim可能通过调控脂肪生成和脂肪分解基因的表达来促进脂质积累。此外，减少脂质积累所造成的暴露于不同的光周期期间DPP是独立的保幼激素。总之，这些结果表明，在DPP期间接收的光周期信号影响DD昆虫中的脂质积累。DD昆虫在DPP期间仍具有一定的监测光周期变化的能力，并且可能参与了滞育准备期间对光周期信号的生理反应的调节。这些结果揭示了光周期信号与滞育准备之间的关系，并可能为如何更好地利用昆虫资源和害虫管理提供新的见解。
Many organisms have evolved a series of adaptions, such as dormancy or diapause in insects that enable them to withstand seasonally adverse conditions. In insects, photoperiodic signals received during the diapause induction phase have irreversible effect on diapause initiation. Insects continue to be exposed to diapause-inducing photoperiod after the diapause induction phase during diapause preparation before they enter diapause. However, how photoperiodic signals experienced during the diapause preparation phase (DPP) regulate diapause remains largely unclear. In this paper, we investigate this in the cabbage beetleColaphellus bowringi. The cabbage beetle is in many respects an ideal experimental model in which to investigate the effect of photoperiodic signals on the DPP because it has facultative reproductive diapause induced by long-day length and has differentiable diapause induction and preparation phases. We found that the lipid content of female cabbage beetles decreased after diapause-destined (DD) individuals were exposed to a diapause-inhibiting photoperiod during the DPP. Two circadian clock negative regulators,perandtim, were probably involved in the photoperiodic response of beetles during the DPP.Perandtimpresented obvious oscillation of circadian rhythm and photoperiodic response during the DPP in DD females and knock-down of these genes in DD females caused their lipid content to decrease.Perandtimprobably promote lipid accumulation by regulating the expression of genes that regulate lipogenesis and lipolysis. Moreover, decreased lipid accumulation caused by exposure to different photoperiods during the DPP was independent of juvenile hormone. In summary, these results suggest that photoperiodic signals received during the DPP influence lipid accumulation in DD insects. DD insects still have some ability to monitor photoperiodic changes during the DPP andperandtimare probably involved in regulating physiological responses to photoperiodic signals during diapause preparation. These results shed light on the relationship between photoperiodic signals and diapause preparation, and may provide new insights on both how to better utilize insects as resources and for pest management.