A putative maize zinc-finger protein gene, ZmAN13, participates in abiotic stress response
A putative maize zinc-finger protein gene, ZmAN13, participates in abiotic stress response
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DOI：
10.1007/s11240-011-9962-2
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发表时间：
2011-06
期刊：
影响因子：
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通讯作者：
N. Xuan;Ying Jin;Hongwei Zhang;Yuanhong Xie;Yunjun Liu;Guoying Wang
中科院分区：
文献类型：
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作者：
N. Xuan;Ying Jin;Hongwei Zhang;Yuanhong Xie;Yunjun Liu;Guoying Wang
玉米 ZnF-AN1 基因家族已进行系统发育研究。在这里，我们试图表征 ZmAN13 的可能功能，ZmAN13 是玉米 ZnF-AN1 基因家族的推定成员。结果表明，ZmAN13在玉米基因组中只有一个拷贝。 ZmAN13在叶片中的表达高于其他器官，并受冷和脱落酸(ABA)诱导，但受NaCl下调。与GFP融合的ZmAN13蛋白定位于拟南芥原生质体和洋葱表皮细胞的细胞质和细胞核中，尽管在酵母系统中未检测到转录因子活性。酵母两种杂交实验表明，ZmAN13 的保守 A20 和 AN1 结构域彼此相互作用。此外，ZmAN13 的保守 A20 结构域与自身相互作用，但 AN1 结构域不能。与野生型相比，拟南芥中 ZmAN13 的过表达赋予了其对寒冷的耐受性，但在种子萌发和幼苗阶段增加了其对盐和干旱的敏感性。
The maize ZnF-AN1 gene family has been phylogenetically studied. Here, we tried to characterize the possible function ofZmAN13, a putative member of the maize ZnF-AN1 gene family. The results showed thatZmAN13had only one copy in the maize genome. The expression ofZmAN13was higher in leaves than other organs and was induced by cold and abscisic acid (ABA), but down-regulated by NaCl.ZmAN13protein fused to GFP was localized in cytoplast and also in the nucleus in bothArabidopsisprotoplast and onion epidermic cells although no transcription factor activity was detected in the yeast system. Yeast two hybrid experiments demonstrated that the conserved A20 and AN1 domains of theZmAN13interacted with each other. Moreover, the conserved A20 domain of theZmAN13interacted with itself, but the AN1 domain could not. Overexpression ofZmAN13inArabidopsisconferred tolerance to cold, but increased their salt and drought sensitivity at seed germination and seedling stage compared to the wild-type.