Binding site of MraZ transcription factor in Mollicutes
Binding site of MraZ transcription factor in Mollicutes
复制标题
DOI：
10.1016/j.biochi.2016.02.016
复制
发表时间：
2016-06-01
期刊：
影响因子：
3.9
通讯作者：
Govorun, V. M.
中科院分区：
文献类型：
--
作者：
Fisunov, G. Y.;Evsyutina, D. V.;Govorun, V. M.
柔膜菌（支原体）的特征是基因表达的已知调节因子的显著丢失。在这里，我们确定了识别位点的MraZ-家族调节器的鸡毒支原体，这是保守的许多物种的不同分支内类柔膜菌。MraZ结合位点为AAAGTG [T/G]，在mraZ基因启动子中形成一系列结构为（AAAGTG[T/G]N-3）（k）的同向重复序列，其中k = 3的频率最高。MraZ作为八聚体复合物与单个重复序列结合。MraZ还可以结合单个结合位点或一系列具有不同间隔区长度（2-4 nt）的重复序列;因此，它可能在柔膜菌中调节多个操纵子中发挥作用。In M.在鸡毒败血症中，MraZ充当转录激活因子。MraZ的过表达导致细胞的中度分裂和聚集体的形成，可能是由于不完全的胞质分裂。(C)2016作者由Elsevier B. V.出版，这是CC BY-NC-ND许可下的开放获取文章。
Mollicutes (mycoplasmas) feature a significant loss of known regulators of gene expression. Here, we identified the recognition site of the MraZ-family regulator of Mycoplasma gallisepticum, which is conserved in many species of different clades within class Mollicutes. The MraZ binding site is AAAGTG [T/G], in the promoter of mraZ gene it forms a series of direct repeats with a structure (AAAGTG[T/G]N-3)(k), where k = 3 most frequently. MraZ binds to a single repeat as an octamer complex. MraZ can also bind a single binding site or a series of repeats with different spacer lengths (2-4 nt); thus, it may play a role in the regulation of multiple operons in Mollicutes. In M. gallisepticum, MraZ acts as a transcriptional activator. The overexpression of MraZ leads to moderate filamentation of cells and the formation of aggregates, likely as a result of incomplete cytokinesis. (C) 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license.