Ethanol-induced damage in rat testis: oxidative stress-mediated germ cell apoptosis and vacuolization in Sertoli cells.
Ethanol-induced damage in rat testis: oxidative stress-mediated germ cell apoptosis and vacuolization in Sertoli cells.
复制标题
乙醇诱导的大鼠睾丸损伤：氧化应激介导的生殖细胞凋亡和支持细胞中的空泡化。
作者：
Eid N;Ito Y;Otsuki Y.
我们饶有兴趣地阅读了维吾尔等人(2014)的文章：Quercetin和FISH n-3脂肪酸对乙醇诱导的大鼠睾丸损伤的影响，最近发表在Andrelogia上。作者发现，槲皮素和鱼n-3脂肪酸可减轻慢性乙醇诱导的睾丸生殖细胞凋亡和氧化应激，并增强抗氧化防御系统。几年前，我们报道了慢性乙醇处理大鼠(ETRs)睾丸生殖细胞凋亡的增加，基于TUNEL方法和电子显微镜，以及通过Fas-FasL死亡受体和线粒体信号通路增加caspase-3、-8和-9的活性(Eid等人，2002；Otsuki，2004)。ETRs生殖细胞的这种凋亡增加可能与睾丸萎缩和不育有关。但有一些观点值得维吾尔等人解释。关于他们文章中的形态观察(维吾尔等人，2014年)。首先，在图3中。1、面板c(乙醇+槲皮素组)和面板d(乙醇+n-3脂肪酸组)，两个面板显示相同的图像！！这种情况怎么会发生在不同动物的两个不同的睾丸中？同样的问题在图3中重复出现。2其中两张显示TUNEL阳性生殖细胞的图片在实验组的c和d组中是相同的！！这些重复的图像可能是偶然的，应该通过解释进行更正。第二，在无花果的传说中。1、作者提到，S是Sertoli cell的缩写，而V表示液泡；遗憾的是，图中没有清楚地显示这些缩写！第三，在结果节中，手稿与图有关。1、作者提到ETR的间质细胞有萎缩；在这种低倍镜下可以观察到什么样的萎缩？间质细胞萎缩是什么意思？维吾尔等人(2014)提到，在ETRS睾丸的生精小管中存在空泡化；但没有讨论这种空泡化的位置和性质。因此，用透射电子显微镜检测和分析慢性酒精处理大鼠睾丸的形态改变和空泡化是有用的。这些空泡可能是支持细胞吞噬后生殖细胞丢失的部位。
We read with interest the article by Uygur et al.(2014): effects of quercetin and fish n-3 fatty acids on testicular injury induced by ethanol in rats, published recently in Andrologia. The authors found that quercetin and fish n-3 fatty acids mitigated chronic ethanol-induced testicular germ cell apoptosis and oxidative stress and enhanced antioxidant defence system. Several years ago, we reported the elevation of testicular germ cell apoptosis in chronic ethanol-treated rats (ETRs) based on TUNEL method, electron microscopy and elevated activity of caspase-3,-8 and-9 via Fas–FasL death receptor and mitochondrial signalling pathways (Eid et al., 2002; Otsuki, 2004). This enhanced apoptosis in germ cells of ETRs may be involved in testicular atrophy and infertility.However, there are some points that deserve explanation by Uygur et al. regarding the morphological observations in their article (Uygur et al., 2014). First, in fig. 1, panel c (ethanol+ quercetin group) and panel d (ethanol+ n-3 fatty acid group), the same image was shown in both panels!! How could this happen in two different testes of different animals? The same problem was repeated in fig. 2 where two images showing TUNEL-positive germ cells were the same in panels c and d of experimental groups!! These repeated images, probably accidental, should be corrected with explanation. Second, in the legend of fig. 1, the authors mentioned that S is abbreviation of Sertoli cells, while V indicates vacuole; unfortunately, the figure did not show clearly these abbreviations! Third, in the results, section of the manuscript and related to fig. 1, the authors mentioned that there was atrophy in Leydig cells of ETRs; What kind of atrophy could be observed at this low magnification? What is the meaning of Leydig cell atrophy? Uygur et al.(2014) mentioned that there is vacuolization in seminiferous tubules of ETRs testes; however, the location and nature of this vacuolization were not discussed. Therefore, it may be useful to use transmission electron microscopy for detection and analysis of morphological alterations and vacuolization in the testes of chronic ethanol-treated rats. These vacuoles may be the sites of germ cell loss after phagocytosis by Sertoli cells