PCR assay for identification of Anopheles quadriannulatus species B from Ethiopia and other sibling species of the Anopheles gambiae complex
PCR assay for identification of Anopheles quadriannulatus species B from Ethiopia and other sibling species of the Anopheles gambiae complex
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DOI：
10.1046/j.1365-2915.2002.00354.x
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发表时间：
2002-06-01
影响因子：
1.9
通讯作者：
Coetzee, M
中科院分区：
文献类型：
--
作者：
Fettene, M;Koekemoer, LL;Coetzee, M
四环按蚊（Anopheles quadriannulatus（Theobald））的同胞种A和B被认为是非洲热带蚊冈比亚按蚊Giles复合体的异地成员。物种A代表An。quadriannulatus sensu stricto，广泛分布于南部非洲，而An. quadriannulatus种B产于埃塞俄比亚。由于鉴别的困难，安氏杆菌的分布。广义上的quadriannulatus仍然知之甚少。细胞分类学和标准的DNA聚合酶链反应（PCR）试验不能区分A和B种。四环带的通过优化标准PCR测定（Scott et al.，1993年），为识别的成员。gambiae复合体的DNA序列中，分别鉴定出153 bp和900 bp的判别片段。quadriannulatus物种B，而来自南非的物种A仅扩增了153 bp片段。因此，这种改良的PCR测定可用于区分An的A种和B种。四环带公司以及安的其他成员冈比亚情结。
Sibling species A and B of Anopheles quadriannulatus (Theobald) are recognized as allopatric members of the Anopheles gambiae Giles complex of Afrotropical mosquitoes (Diptera: Culicidae). Species A represents An. quadriannulatus sensu stricto, widespread in southern Africa, whereas An. quadriannulatus species B occurs in Ethiopia. Because of difficulty of identification, distribution of An. quadriannulatus sensu lato remains poorly known. Cytotaxonomy and the standard DNA polymerase chain reaction (PCR) assay do not distinguish between species A and B of An. quadriannulatus. By optimizing the standard PCR assay (Scott et al., 1993) for identification of members of the An. gambiae complex, we identified two discriminant fragments of 153 bp and 900 bp from DNA of An. quadriannulatus species B, whereas only the 153 bp fragment was amplified for species A from South Africa. This modified PCR assay can therefore be used to distinguish between species A and B of An. quadriannulatus s.l. as well as other members of the An. gambiae complex.