The effect of graded hypoxia on the hippocampal slice: an in vitro model of the ischemic penumbra.
The effect of graded hypoxia on the hippocampal slice: an in vitro model of the ischemic penumbra.
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分级缺氧对海马切片的影响：缺血半暗带的体外模型。
作者：
Schiff,SJ;Somjen,GG
浸没的海马切片在29℃下暴露于中度或轻度缺氧30分钟，然后重新充氧。无论哪种程度的缺氧，突触传递都会以相同的速度丧失，但轻度缺氧后的恢复速度更快。突触传递的过度兴奋是中度缺氧后的一个持久特征，但在轻度缺氧后是短暂的；轻度缺氧后，突触传递的强度最终恢复正常。中度缺氧期间细胞外钙没有变化。切片的细胞外 pH 值总是比浴液更酸； pH值因中度和轻度缺氧而进一步下降。中度缺氧期间细胞外钾的增加比轻度缺氧期间更多，并且中度缺氧后一段快速钾摄取期也更明显。细胞外直流电位在缺氧期间表现出小的正向变化，在轻度缺氧期间更是如此。这些实验表明，在轻度缺氧的脑组织中，突触功能可以被可逆地抑制，而不会导致神经元严重去极化；此外，缺氧后过度兴奋的程度和持续时间与缺氧程度和细胞向环境中释放 K+ 的程度相关。
Submerged hippocampal slices were exposed to 30 minutes of moderate or mild hypoxia at 29 degrees C and then reoxygenated. Synaptic transmission was lost at the same rate in response to either grade of hypoxia, but recovery was faster following mild hypoxia. Hyperexcitability of synaptic transmission was a lasting feature following moderate hypoxia, but it was transient following mild hypoxia; after mild hypoxia the strength of synaptic transmission eventually returned to normal. Extracellular calcium did not change during moderate hypoxia. The extracellular pH of slices was always more acid than the bath; pH decreased further in response to both moderate and mild hypoxia. Extracellular potassium increased more during moderate than during mild hypoxia, and a period of rapid potassium uptake was also more pronounced following moderate hypoxia. Extracellular DC potential demonstrated a small positive shift during hypoxia, more so during mild hypoxia. These experiments suggest that synaptic function can be reversibly suppressed in mildly hypoxic brain tissue without severe depolarization of neurons; in addition, the degree and duration of posthypoxic hyperexcitability are correlated with the degree of hypoxia and the magnitude of the release of K+ from cells into their environment.
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