Micromanipulation of sperm by a laser generated optical trap.
Micromanipulation of sperm by a laser generated optical trap.
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DOI：
10.1016/s0015-0282(16)53057-x
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发表时间：
1989-11
影响因子：
6.7
通讯作者：
Y. Tadir;W. Wright;Omid Vafa;T. Ord;R. Asch;M. Berns
中科院分区：
文献类型：
--
作者：
Y. Tadir;W. Wright;Omid Vafa;T. Ord;R. Asch;M. Berns
低功率激光束的辐射压力产生的力会产生一个光学陷阱，可以用来操纵精子。我们研究了光学陷阱对精子活力的影响。将一束激光耦合到常规显微镜上，通过物镜聚焦到观察面上。精子被捕获在诱捕器中，并由喜悦手杖控制的机动平台操纵。在不同的曝光时间后，用计算机图像处理器分析速度和图案。当暴露在捕捉器中30秒或更短时间时，精子速度有微小的变化。曝光45秒后，平均线速度逐渐降低。这种光学显微操纵器也可能用于研究单个精子产生的力，并评估药物对活力的影响。
The force generated by the radiation pressure of a low power laser beam induces an optical trap which may be used to manipulate sperm. We studied the effect of the optical trap on sperm motility. A Nd:YAG laser beam was coupled to a conventional microscope and focused into the viewing plane by the objective lens. Sperm were caught in the trap and manipulated by a joy stick controlled motorized stage. After different exposure periods, the velocity and patterns were analysed by a computerized image processor. There were minor changes in sperm velocity when exposed to the trap for 30 seconds or less. A gradual decrease in the mean linear velocity was observed after 45 seconds of exposure. This optical micromanipulator may also be useful for studying the force generated by a single spermatozoa and evaluating the influence of drugs on motility.