A prominent role for the CBF cold response pathway in configuring the low-temperature metabolome of Arabidopsis
A prominent role for the CBF cold response pathway in configuring the low-temperature metabolome of Arabidopsis
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DOI：
10.1073/pnas.0406069101
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发表时间：
2004-10-19
影响因子：
11.1
通讯作者：
Thomashow, MF
中科院分区：
文献类型：
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作者：
Cook, D;Fowler, S;Thomashow, MF
拟南芥CBF冷反应途径在冷驯化过程中起着重要作用，冷驯化是植物对低非冻结温度的反应，从而提高植物的抗冻性。在这里，我们研究了拟南芥代谢组在低温下发生的变化，并评估了CBF冷反应途径在这些修饰中的作用。在434种代谢产物中，有325种（75%）在拟南芥Wassilewskija-2（Ws-2）植物中对低温的反应有所增加。在这325种代谢产物中，256种（79%）也在非驯化的Ws-2植物中增加，以响应C-重复/脱水反应元件结合因子（CBF）3的过度表达。广泛的冷诱导的变化也发生在拟南芥佛得角群岛-1（Cvi-1）植物的代谢组中，这被认为是较低的抗冻性比Ws-2植物。然而，Cvi-1中CBF 1、CBF 2、CBF 3和CBF靶向基因的低温表达远低于Ws-2植物，并且Cvi-1植物的低温代谢物组中受CBF 3过表达影响的代谢物被耗尽。总之，这些结果表明，拟南芥的代谢组是广泛重新配置在响应低温，CBF冷反应途径在这个过程中有一个突出的作用。
The Arabidopsis CBF cold response pathway has a central role in cold acclimation, the process whereby plants increase in freezing tolerance in response to low nonfreezing temperatures. Here we examined the changes that occur in the Arabidopsis metabolome in response to low temperature and assessed the role of the CBF cold response pathway in bringing about these modifications. Of 434 metabolites monitored by GC-time-of-flight MS, 325 (75%) were found to increase in Arabidopsis Wassilewskija-2 (Ws-2) plants in response to low temperature. Of these 325 metabolites, 256 (79%) also increased in nonacclimated Ws-2 plants in response to overexpression of C-repeat/dehydration responsive element-binding factor (CBF)3. Extensive cold-induced changes also occurred in the metabolome of Arabidopsis Cape Verde Islands-1 (Cvi-1) plants, which were found to be less freezing tolerant than Ws-2 plants. However, low-temperature-incluced expression of CBF1, CBF2, CBF3, and CBF-targeted genes was much lower in Cvi-1 than in Ws-2 plants, and the low-temperature metabolome of Cvi-1 plants was depleted in metabolites affected by CBF3 overexpression. Taken together, the results indicate that the metabolome of Arabidopsis is extensively reconfigured in response to low temperature, and that the CBF cold response pathway has a prominent role in this process.