Diverse Fusarium solani isolates colonise agricultural environments in Ethiopia
Diverse Fusarium solani isolates colonise agricultural environments in Ethiopia
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DOI：
10.1007/s10658-008-9422-y
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发表时间：
2009-07-01
影响因子：
1.8
通讯作者：
Wingfield, B. D.
中科院分区：
文献类型：
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作者：
Bogale, M.;Steenkamp, E. T.;Wingfield, B. D.
茄病镰刀菌（Fusarium solani）是一种侵染许多不同属植物的真菌病原体。它代表两种镰刀菌属之一。通常从埃塞俄比亚的农业土壤和植物组织中分离。测定F.我们使用扩增片段长度多态性（AFLP）以及翻译延伸因子1 α（TEF-1 α）和β-微管蛋白基因的核苷酸序列研究了43个分离株。TEF-1 α序列来自GenBank，代表先前描述的F.茄-红球菌赤壳菌复合体也包括在内用于比较目的。TEF-1 α数据的系统发育分析将分离物分成三组，对应于该真菌的三个先前描述的进化枝（进化枝1-3）。埃塞俄比亚分离物聚集成一个对应于进化枝3的组。TEF-1 α、β-微管蛋白和AFLP进一步将埃塞俄比亚分离株分成许多簇和显然是新的系统发育谱系。虽然这些谱系和集群的生物学和生态学意义尚不清楚，但我们的数据表明，埃塞俄比亚农业环境中有丰富的F。茄属H.红球菌复合体
Fusarium solani is a fungal pathogen that infects many different genera of plants. It represents one of the two Fusarium spp. commonly isolated from agricultural soils and plant tissues in Ethiopia. To determine the diversity of F. solani in Ethiopia, we studied 43 isolates using Amplified Fragment Length Polymorphism (AFLP) and nucleotide sequences of the Translation Elongation Factor 1 alpha (TEF-1 alpha) and beta-tubulin genes. TEF-1 alpha sequences from GenBank, representing previously described species and clades of the F. solani-Haematonectria haematococca complex, were also included for comparative purposes. Phylogenetic analyses of the TEF-1 alpha data separated the isolates into three groups corresponding with the three previously described clades (Clades 1-3) for this fungus. The Ethiopian isolates aggregated into one group corresponding to Clade 3. TEF-1 alpha, beta-tubulin and AFLPs further separated the Ethiopian isolates into a number of clusters and apparently novel phylogenetic lineages. Although the biological and ecological significance of these lineages and clusters is unclear, our data show that the Ethiopian agricultural environment is rich in species and lineages of the F. solani-H. haematococca complex.