Target Enrichment and Extensive Population Sampling Help Untangle the Recent, Rapid Radiation of Oenothera Sect. Calylophus
Target Enrichment and Extensive Population Sampling Help Untangle the Recent, Rapid Radiation of Oenothera Sect. Calylophus
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DOI：
10.1093/sysbio/syac032
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发表时间：
2022-06-10
影响因子：
6.5
通讯作者：
Wickett, Norman J.
中科院分区：
文献类型：
--
作者：
Cooper, Benjamin J.;Moore, Michael J.;Wickett, Norman J.
月见草属Calylophus（Oenothera sect. Calylophus）是晚报春花科（Onagraceae）的一个北美类群，由13个已知的类群组成，其进化历史可能包括蜜蜂授粉的独立起源、土壤特有性和永久易位杂合性。像其他群体辐射相对较近和迅速，分类单元内的界限月见草组。在这项研究中，我们使用目标富集，侧翼非编码区，基因树/物种树的方法，测试基因流修改的目标富集数据，和形态计量学分析，以重建系统发育假说，评估目前的分类群circumscarpus，并研究在月见草组的字符进化。由于组。Calylophus包括一个分支，一个相对有限的地理范围，我们能够广泛的样品在整个范围内的地理，土壤和形态多样性的组。我们发现，外显子和侧翼非编码区的组合，导致物种关系的改善支持。我们重建了一些加入潜在的混合起源，并指出，如果不考虑杂交等过程，推断的进化过渡的数量可能会被人为夸大。我们从祖先天蛾授粉、石膏上的土壤专业化和永久易位杂合性中恢复了蜜蜂授粉的多个进化起源的有力证据。本研究应用了新出现的技术，以及密集的种下采样和形态分析，有效地重建了快速辐射的历史。[石膏特有性;月见草组;柳叶菜科;花粉基因组学;传粉者转移;最近辐射;目标富集。]
Oenothera sect. Calylophus is a North American group of 13 recognized taxa in the evening primrose family (Onagraceae) with an evolutionary history that may include independent origins of bee pollination, edaphic endemism, and permanent translocation heterozygosity. Like other groups that radiated relatively recently and rapidly, taxon boundaries within Oenothera sect. Calylophus have remained challenging to circumscribe. In this study, we used target enrichment, flanking noncoding regions, gene tree/species tree methods, tests for gene flow modified for target-enrichment data, and morphometric analysis to reconstruct phylogenetic hypotheses, evaluate current taxon circumscriptions, and examine character evolution in Oenothera sect. Calylophus. Because sect. Calylophus comprises a clade with a relatively restricted geographic range, we were able to extensively sample across the range of geographic, edaphic, and morphological diversity in the group. We found that the combination of exons and flanking noncoding regions led to improved support for species relationships. We reconstructed potential hybrid origins of some accessions and note that if processes such as hybridization are not taken into account, the number of inferred evolutionary transitions may be artificially inflated. We recovered strong evidence for multiple evolutionary origins of bee pollination from ancestral hawkmoth pollination, edaphic specialization on gypsum, and permanent translocation heterozygosity. This study applies newly emerging techniques alongside dense infraspecific sampling and morphological analyses to effectively reconstruct the recalcitrant history of a rapid radiation. [Gypsum endemism; Oenothera sect. Calylophus; Onagraceae; phylogenomics; pollinator shift; recent radiation; target enrichment.]