THE SEQUENCE 5'-AAUAAA-3' FORMS PART OF THE RECOGNITION SITE FOR POLYADENYLATION OF LATE SV40 MESSENGER-RNAS
THE SEQUENCE 5'-AAUAAA-3' FORMS PART OF THE RECOGNITION SITE FOR POLYADENYLATION OF LATE SV40 MESSENGER-RNAS
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DOI：
10.1016/0092-8674(81)90521-3
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
SHENK, T
中科院分区：
文献类型：
--
作者：
FITZGERALD, M;SHENK, T
缺失突变对晚期SV-40 mRNA的多聚腺苷酸化有三种影响。第一类突变体在5“-AAUAAA-3”和正常poly（A）位点之间缺少片段（3-14 bp [碱基对]）。这些突变体在野生型位点下游的新位点产生多聚腺苷酸化的mRNA。当缺失变大时，多聚腺苷酸位点向下游移动得更远;因此，多聚腺苷酸化总是发生在距离AAUAAA序列11-19个核苷酸的范围内。第二类突变体在AAUAAA序列和mRNA编码区之间缺少片段[12-30 bp]。仅研究了这些突变体中的一个（dl 1457，-12bp）的聚（A）位点。在这种情况下，AAUAAA和poly（A）位点之间的空间关系被改变。dl 1457突变体产生一类在AAUAAA序列之后的第一CA处聚腺苷酸化的mRNA，以及进一步下游聚腺苷酸化的其他mRNA。包括AAUAAA序列的16 bp缺失防止多聚腺苷酸添加。
Three effects of deletion mutations were observed on polyadenylation of late SV-40 mRNA. The 1st class of mutants lack segments (3-14 bp [base pairs] between the 5''-AAUAAA-3'' and normal poly(A) site. These mutants produce mRNA polyadenylated at new sites, downstream from the wild-type site. The poly(A) site is moved farther downstream as the deletions become larger; as a result, polyadenylation always occurs within an 11-19 nucleotide range from the AAUAAA sequence. The 2nd class of mutants lack segments [12-30 bp] between the AAUAAA sequence and the coding region of the mRNA. The poly(A) site for only one of these mutants was studied (dl1457, -12 bp). In this case, the spatial relationship between AAUAAA and poly(A) site is altered. The dl1457 mutant produces a class of mRNA polyadenylated at the 1st CA following the AAUAAA sequence, as well as other mRNA polyadenylated further downstream. A 16 bp deletion that includes the AAUAAA sequence prevents poly(A) addition.