Soybean lecithin-based extender preserves spermatozoa membrane integrity and fertilizing potential during goat semen cryopreservation
Soybean lecithin-based extender preserves spermatozoa membrane integrity and fertilizing potential during goat semen cryopreservation
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DOI：
10.1016/j.theriogenology.2014.12.012
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发表时间：
2015-04-01
期刊：
影响因子：
2.8
通讯作者：
Berlinguer, Fiammetta
中科院分区：
文献类型：
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作者：
Chelucci, Sara;Pasciu, Valeria;Berlinguer, Fiammetta
大豆卵磷脂可能是一种较好的替代卵黄的畜禽精液冷冻保存材料。但其对精子功能特性的影响还有待进一步研究。将5只萨尔达雄鹿的精液冷冻保存在tris扩展剂和不同添加量的甘油（4% v:v）中。在初步实验中，对不同浓度的大豆卵磷脂（1% ~ 6% wt/vol）进行了测试，结果表明，在精子活力、精子运动和快速精子百分比以及解冻后DNA完整性方面，浓度为1%的大豆卵磷脂最有效。在第二个实验中，精液在不添加任何添加物（EXT）的tris扩展器中冷冻，添加1%卵磷脂（EXT LC）和20%蛋黄（EXT EY）。并与市售扩展剂进行了效果比较。EXT EY在冷冻和解冻后的活力和运动参数最高（P < 0.0001）。细胞内ATP浓度无显著差异。其他分子特征表明，与ExT LC中冷冻保存的精子相比，ExT EY中精子功能受到影响，DNA和顶体完整性降低（P < 0.05），脂质过氧化水平升高（P < 0.0001）。体外受精试验结果表明，EXT LC能更好地保存精子功能，受精率高于其他培养基（EXT LC为66.2 +/- 4.5%，EXT、EXT EY和商业扩展剂为32.7 +/- 4.5%,38.7 +/- 4.5%,39.6 +/- 5.2%,P < 0.01）。本研究表明卵磷脂可以被认为是山羊精液冷冻保存中蛋黄的合适替代品，因为卵磷脂可以保证较高的受精率，并且可以更好地保护膜免受冷休克的损伤。(C) 2015爱思唯尔公司版权所有。
Soybean lecithin may represent a suitable alternative to egg yolk for semen cryopreservation in livestock species. However, additional studies are needed to elucidate its effects on spermatozoa functional properties. Semen collected from five Sarda bucks was cryopreserved in Tris-based extender and glycerol (4% v:v) with different supplementations. In a preliminary experiment, different soybean lecithin concentrations were tested (1%-6% wt/vol) and results in terms of viability, percentages of progressive motile and rapid spermatozoa, and DNA integrity after thawing showed that the most effective concentration was 1%. In the second experiment, semen was frozen in a Tris-based extender with no supplementation (EXT), with 1% lecithin (EXT LC), and 20% egg yolk (EXT EY). The effectiveness of these extenders was also compared with a commercial extender. The EXT EY led to the highest viability and motility parameters after freezing and thawing (P < 0.0001). No significant differences were observed in intracellular ATP concentrations. Additional molecular features revealed that sperm functionality was affected in ExT EY, as demonstrated by lower DNA and acrosome integrity (P < 0.05), and higher lipid peroxidation compared with spermatozoa cryopreserved in EXT LC (P < 0.0001). Results obtained in the heterologous in vitro fertilization test showed that EXT LC better preserved spermatozoa functionality, as demonstrated by the higher fertilization rates compared with the other media (66.2 +/- 4.5% for EXT LC vs. 32.7 +/- 4.5%, 38.7 +/- 4.5%, 39.6 +/- 5.2% for EXT, EXT EY, and commercial extender; P < 0.01). The present study demonstrated that lecithin can be considered as a suitable alternative to egg yolk in goat semen cryopreservation, because it ensures higher fertilization rates and a better protection from membrane damage by cold shock. (C) 2015 Elsevier Inc. All rights reserved.