Regeneration of optic nerve fibers with unoprostone, a prostaglandin-related antiglaucoma drug, in adult cats.
Regeneration of optic nerve fibers with unoprostone, a prostaglandin-related antiglaucoma drug, in adult cats.
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使用乌诺前列酮（一种前列腺素相关的抗青光眼药物）对成年猫进行视神经纤维的再生。
DOI：
10.1007/s10384-013-0282-4
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Watanabe M.
中科院分区：
文献类型：
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作者：
Sagawa H;Terasaki H;Nakanishi K;Tokita Y;Watanabe M.
目的观察乌诺前列酮对体外培养的成年猫视神经轴突生长和视神经节细胞轴突再生的影响。方法将乌诺前列酮或其主要代谢产物M1溶于DMSO或聚山梨醇酯中培养14天，检测Tau-1阳性神经突和抗胶质细胞酸性蛋白抗体标记的胶质突起的数量和长度。视神经被压碎后，将乌诺前列酮注入玻璃体和压碎部位。在第12天，将麦胚凝集素结合的辣根过氧化物酶注射到玻璃体中以顺行标记再生的轴突。在第14天，切除视神经并纵向切片。结果在3 μM乌诺前列酮和3 μM M1中，培养的视网膜碎片突起的数量最多。在3 μM乌诺前列酮和3 μM M1时，神经突长度也最长，其中未检测到胶质突起。将玻璃体液中的最终浓度3 μM乌诺前列酮注射到玻璃体和压碎部位后，视神经纤维再生并延伸到压碎部位以外。相比之下，几乎没有纤维延伸超出压碎部位后，注射磷酸盐缓冲salines.ConclusionsThe结果表明，玻璃体内注射乌诺前列酮促进再生破碎的视神经纤维在成年猫。
PurposeWe investigated the effects of unoprostone on neurite extension of cultured retinal pieces and axonal regeneration of retinal ganglion cells in the crushed optic nerve of adult cats.MethodsThe retinal pieces were cultured with unoprostone or its primary metabolite, M1, dissolved in DMSO or polysorbate for 14 days, and the number and length of Tau-1-positive neurites and glial processes labeled with anti-glial fibrillary acidic protein antibodies were examined. After the optic nerve was crushed, unoprostone was injected into the vitreous body and the crushed site. On day 12, wheat germ agglutinin-conjugated horseradish peroxidase was injected into the vitreous body to anterogradely label the regenerated axons. On day 14, the optic nerve was excised and longitudinally sectioned. After peroxidase reaction, the number of axons regenerating beyond the crush site was examined.ResultsThe greatest number of neurites protruded from the cultured retinal pieces in 3 μM unoprostone and 3 μM M1. The neurite length was also the longest at 3 μM unoprostone and 3 μM M1, in which no glial processes were detected. After injections of 3 μM unoprostone, the final concentration in the vitreous humor, into the vitreous body and the crush site, the optic nerve fibers regenerated and extended beyond the crush site. In contrast, almost no fibers extended beyond the crush site after injection of phosphate-buffered saline.ConclusionsThe results indicate that intravitreal injection of unoprostone promotes regeneration of crushed optic nerve fibers in adult cats.