New insights into the entrance of Perkinsus olseni in the Manila clam, Ruditapes philippinarum
New insights into the entrance of Perkinsus olseni in the Manila clam, Ruditapes philippinarum
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关于 Perkinsus olseni 进入菲律宾蛤蜊（Ruditapes philippinarum）的新见解
DOI：
10.1016/j.jip.2018.03.005
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发表时间：
2018
影响因子：
3.4
通讯作者：
Itoh Naoki
中科院分区：
文献类型：
--
作者：
Wang Yu;Yoshinaga Tomoyoshi;Itoh Naoki
为了解奥氏帕金斯（Perkinsus olsenis）与其寄主软体动物菲律宾蛤仔（Ruditapes philippinarum）之间的相互作用，本研究着重探讨了该寄生虫入侵宿主的过程，特别是游动孢子转化为滋养体的机制。我们把马尼拉蛤暴露在水中。olsenizoospores，然后定期定量寄生虫强度在各种宿主器官和组织。我们检测到大量的寄生虫细胞内的鳃和唇须的主机蛤从早期到最后阶段，适度内地幔和消化器官，但在血淋巴，脚和内收肌的数量很少。结果表明，P. Olseniizoospores暴露于鳃和唇须提取物转化成滋养体的效率比暴露于其他组织时更高;当游动孢子暴露于加热的器官提取物时，没有观察到这种转化。我们的研究结果表明，在转化的宿主分子的参与ofP。olsenizoospores，导致最初的感染主要在鳃和唇须的主机蛤。
In order to understand interactions betweenPerkinsus olseniand its host mollusk species Manila clamRuditapes philippinarum, this study focused on invasion processes of the parasite, particularly the mechanisms of zoospore transformation to trophozoites in its portal entry into the host. We exposed Manila clam toP. olsenizoospores, then periodically quantified parasite intensity in various host organs and tissues. We detected large numbers of parasite cells within gills and labial palps of the host clam from the early to the final stages, moderately within mantle and digestive organs but low numbers within hemolymph, foot and adductor muscles. Our results suggest thatP. olsenifirst invades the gills and labial palps of the host clam with limited translocation throughout the host body via the host’s circulatory system until 12 days post exposure to zoospores.P. olsenizoospores exposed to extracts of gills and labial palps transformed into trophozoites more efficiently than they did when exposed to other tissues; this transformation was not observed when zoospores were exposed to heated organ extracts. Our results suggest the involvement of a host molecule in the transformation ofP. olsenizoospores, leading to initial infection primarily within gills and labial palps of the host clam.