Protective effect of Alhagi sparsifolia against acetaminophen-induced liver injury in mice
Protective effect of Alhagi sparsifolia against acetaminophen-induced liver injury in mice
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疏叶骆驼刺对对乙酰氨基酚所致小鼠肝损伤的保护作用
DOI：
10.4314/tjpr.v17i4.11
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发表时间：
2018-04-01
影响因子：
0.6
通讯作者：
Zhang, Xiaoying
中科院分区：
文献类型：
--
作者：
Aierken, Aili;Jiang, Zhihui;Zhang, Xiaoying
目的：研究骆驼刺提取物对乙酰氨基酚（APAP）诱导的小鼠肝损伤的保护作用。研究方法：将三种剂量的骆驼刺（600、300和150 mg/kg）经口给予各组小鼠，每天一次，持续三天。最后一次给药后1小时，腹腔注射APAP（300 mg/kg）。取肝组织检测血清谷草转氨酶（AST）和丙氨酸转氨酶（ALT）水平作为肝损伤的生物标志物;丙二醛（MDA）;过氧化氢（H2 O2）;谷胱甘肽（GSH）作为肝脏氧化还原的指标;以及使用超氧化物歧化酶（SOD）测定的抗氧化酶活性。采用蛋白质印迹法检测APAP代谢关键酶细胞色素P450 2 E1（CYP 2 E1）的表达。结果如下：与APAP单独给药对照组相比，疏叶骆驼刺组小鼠血清ALT、AST和CYP 2 E1水平明显降低，MDA、H2 O2和SOD水平升高，且呈剂量依赖性。结论：结果表明，骆驼刺对APP诱导的小鼠肝损伤具有保护作用，其机制可能与降低氧化应激和抑制CYP 2 E1表达有关。关键词：骆驼刺，多糖，对乙酰氨基酚，CYP 2 E1，抗氧化
Purpose : To investigate the hepatoprotective effects of Alhagi sparsifolia extract against acetaminophen (APAP)-induced liver injury in mice. Methods : Three doses of Alhagi sparsifolia (600, 300 and 150 mg/kg) were were administered to separate groups of mice orally once a day for three days. One-hour after the last dose, APAP (300 mg/kg) was intraperitoneally injected. Liver tissue was taken and tested for levels of serum aspartate aminotransferase (AST) and alanine transaminase (ALT) as biomarkers of liver injury; malonaldehyde (MDA); hydrogen peroxide (H 2 O 2 ); glutathione (GSH) as an indicator of liver redox; and antioxidant enzyme activity using super oxide dismutase (SOD) assay. Additionally, western blotting was used to measure the expression of protein cytochrome P450 2E1 (CYP2E1) as the key enzyme of APAP metabolism. Results : Blood serum of ALT and AST and levels of CYP2E1 were markedly reduced, while the levels of MDA, H 2 O 2 , and SOD were elevated in a dose-dependent manner in mice treated with Alhagi sparsifolia compared to control group treated with APAP alone. Conclusion : The results demonstrate that Alhagi sparsifolia protects mice liver tissue against APAPinduced hepatic injury partly via decreased oxidative stress and inhibition of CYP2E1 expression. Keywords : Alhagi sparsifolia, Polysaccharide, Acetaminophen, CYP2E1, Antioxidant