The identification and biosynthesis of siderochromes formed by Micrococcus denitrificans.
The identification and biosynthesis of siderochromes formed by Micrococcus denitrificans.
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DOI：
10.1042/bj1460191
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
G. H. Tait
中科院分区：
文献类型：
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作者：
G. H. Tait
1.当在缺乏铁的培养基中有氧和无氧生长以及在高浓度Ca 2+的培养基中无氧生长时，脱氮微球菌分泌三种含邻苯二酚的化合物，这些化合物可以结合铁。2.这些化合物中的一种被鉴定为2，3-二羟基苯甲酸（化合物I），另外两种被初步鉴定为N1 N8-双-（2，3-二羟基苯甲酰基）亚精胺（化合物II）和2-羟基苯甲酰基-N-L-苏氨酰基-N4[N1 N8-双-（2，3-二羟基苯甲酰基）]亚精胺（化合物III）。3.制备化合物III的等摩尔铁络合物;化合物III还与Al 3+、Cr 3+和Co 2+离子形成络合物。4.缺铁生物体的无细胞提取物催化从2，3-二羟基苯甲酸和亚精胺形成化合物II，以及从化合物II、L-苏氨酸和2-羟基苯甲酸形成化合物III;这两种反应都需要ATP和二硫苏糖醇，并且Mg 2+刺激活性。催化化合物II形成的酶体系在pH8.8时具有最佳活性。Fe ~（2+）（35 μ M）、Fe ~（3+）（35 μ M）和Al ~（3+）（65 μ M）对该反应抑制50%。形成化合物III的酶系统在pH 8.6下具有最佳活性。Fe 2+（110 μ M）、Fe 3+（110 μ M）和Al 3+（135 μ M）抑制反应50%。5.形成化合物II需要至少两种蛋白质，而将其转化为化合物III需要另外两种蛋白质。6.这两个系统的活动的变化后，培养成为缺铁。7.当来自缺铁细胞的无细胞提取物与化合物III、琥珀酸盐、NADH和1，10-菲咯啉的铁络合物在N2下孵育时，形成亚铁1，10-菲咯啉。
1. Micrococcus denitrificans excretes three catechol-containing compounds, which can bind iron, when grown aerobically and anaerobically in media deficient in iron, and anaerobically in medium with a high concentration of Ca2+. 2. One of these compounds was identified as 2,3-dihydroxybenzoic acid (compound I), and the other two were tentatively identified as N1N8-bis-(2,3-dihydroxybenzoyl)spermidine (compound II) and 2-hydroxybenzoyl-N-L-threonyl-N4[N1N8-bis-(2,3-dihydroxybenzoyl)]spermidine (compound III). 3. The equimolar ferric complex of compound III was prepared; compound III also forms complexes with Al3+, Cr3+ and Co2+ ions. 4. Cell-free extracts from iron-deficient organisms catalyse the formation of compound II from 2,3-dihydroxybenzoic acid and spermidine, and of compound III from compound II, L-threonine and 2-hydroxybenzoic acid; both reactions require ATP and dithiothreitol, and Mg2+ stimulates activity. The enzyme system catalysing the formation of compound II has optimum activity at pH 8.8 Fe2+ (35muM), Fe3+ (35muM) and Al3+ (65muM) inhibit the reaction by 50 percent. The enzyme system forming compound III has optimum activity at pH 8.6. Fe2+ (110 muM), Fe3+ (110 muM) and Al3+ (135 muM) inhibit the reaction by 50 percent. 5. At least two proteins are required for the formation of compound II, and another two proteins for its conversion into compound III. 6. The changes in the activities of these two systems were followed after cultures became deficient in iron. 7. Ferrous 1,10-phenanthroline is formed when a cell-free extract from iron-deficient cells is incubated with the ferric complex of compound III, succinate, NADH and 1,10-phenanthroline under N2.