The effect of melatonin on the secretion of progesterone in sheep and on the development of ovine embryos in vitro
The effect of melatonin on the secretion of progesterone in sheep and on the development of ovine embryos in vitro
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DOI：
10.1023/a:1014099719034
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发表时间：
2002-03-01
影响因子：
2.2
通讯作者：
Zúñiga, O
中科院分区：
文献类型：
--
作者：
Abecia, JA;Forcada, F;Zúñiga, O
为了确定褪黑激素是否可以改善体内黄体酮的分泌以及其对体外胚胎发育的影响，进行了两个实验。在第一个实验中，在停用孕激素子宫托后 7 天和 10 天，每隔 15 分钟从 5 只母羊采集血样，持续 2 小时。第一个小时构成对照期，以静脉注射 3 杯/(kg bw)(0.75) 褪黑激素结束。第 7 天的所有母羊和第 10 天的三只母羊都表现出对褪黑激素激发的孕酮反应，定义为在治疗后期间至少两个连续样品中血浆孕酮浓度增加到高于治疗前期间值的平均值+2SD。配对 t 检验显示，在第 7 天的攻击前后，褪黑激素对总体血浆孕酮浓度有显着影响（前，0.61+/-0.11；后，0.73+/-0.13 ng/ml；p
Two experiments were carried out in order to determine whether melatonin can improve secretion of progesterone in vivo, and its effect on embryonic development in vitro. In the first experiment, blood samples were collected from 5 ewes at 15 min intervals for 2 h at 7 and 10 days after withdrawal of progestagen pessaries. The first hour constituted a control period, which ended with an intravenous administration of 3 mug/(kg bw)(0.75) melatonin. All the ewes on day 7 and three of the ewes on day 10 showed a progesterone response to melatonin challenge, defined as an increase in the plasma progesterone concentration in at least two consecutive samples during the post-treatment period above the mean+2SD of the values in the pre-treatment period. A paired t-test revealed a significant effect of melatonin on the overall plasma progesterone concentrations before and after the challenge, both on day 7 (pre, 0.61+/-0.11; post, 0.73+/-0.13 ng/ml; p