ONSET OF CELL-SPECIFIC GENE-EXPRESSION IN THE DEVELOPING MOUSE PANCREAS
ONSET OF CELL-SPECIFIC GENE-EXPRESSION IN THE DEVELOPING MOUSE PANCREAS
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DOI：
10.1073/pnas.89.3.1128
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发表时间：
1992-02-01
影响因子：
11.1
通讯作者：
RUTTER, WJ
中科院分区：
文献类型：
--
作者：
GITTES, GK;RUTTER, WJ
发育生物学中的一个中心问题是细胞特异性基因表达的启动及其与形态发生的时间关系。我们将胚胎显微解剖与聚合酶链式反应的敏感度结合起来，以确定胰腺器官发生过程中细胞特异性基因表达的开始。利用每个胚胎中体节数对胎龄的精确分配，我们确定了小鼠发育期间内分泌和外分泌胰腺主要基因的转录起始时间为2-3小时。生长抑素mRNA在整个前肠的10个体节阶段被检测到，这与整个成人肠道中生长抑素产生细胞的存在是一致的。胰岛素和胰升糖素的成熟信使核糖核酸首次出现的时间出奇地早，在胚胎前肠的20体节阶段，并且仅限于10-12小时后胰腺长出的十二指肠区域。相反，外分泌基因转录在胰腺憩室形成24小时后开始。因此，细胞特异性基因在内分泌胰腺中的表达开始于“前形态发生阶段”。胰岛素和胰升糖素的早期表达可能反映了内分泌细胞谱系的启动。
A central question in developmental biology has been the initiation of cell-specific gene expression and its temporal relationship to morphogenesis. We have coupled embryo microdissection with the exquisite sensitivity of the polymerase chain reaction to define the onset of cell-specific gene expression during pancreatic organogenesis. Using the precise assignment of gestational age by the number of somites in each embryo, we determined the onset of transcription of major genes of the endocrine and exocrine pancreas during mouse development to within 2-3 hr. Somatostatin mRNA was detected at the 10-somite stage throughout the foregut, consistent with the presence of somatostatin-producing cells throughout the adult gut. Mature mRNA for insulin and glucagon first appears surprisingly early, at the 20-somite stage in the wall of the embryonic foregut and is restricted to only the area of the duodenum from which the pancreas will arise 10-12 hr later. In contrast, exocrine gene transcription begins 24 hr after formation of the pancreatic diverticulum. Thus cell-specific gene expression in the endocrine pancreas begins in a "premorphogenetic phase." this early expression of insulin and glucagon could reflect the initiation of an endocrine cell lineage.