Changes in pulsed-field gel electrophoresis patterns in clinical isolates of enterohemorrhagic Escherichia coli O157:H7 associated with loss of shiga toxin genes
Changes in pulsed-field gel electrophoresis patterns in clinical isolates of enterohemorrhagic Escherichia coli O157:H7 associated with loss of shiga toxin genes
复制标题
DOI：
10.1007/pl00006771
复制
发表时间：
1999-01-01
影响因子：
2.6
通讯作者：
Watanabe, H
中科院分区：
文献类型：
--
作者：
Murase, T;Yamai, S;Watanabe, H
对肠出血性大肠杆菌（EHEC） O157:H7菌株的xbai酶切DNA片段的脉冲场凝胶电泳（PFGE）显示，在维持或继代培养过程中，与志贺毒素基因丢失相关的70或80 kb片段消失。与志贺毒素序列互补的DNA探针杂交实验显示，亲本菌株的志贺毒素基因位于与毒素阴性衍生物缺失片段相同大小的片段上，这表明EHEC O157:H7的PFGE模式可能由于志贺毒素基因的缺失而发生变化，这可能与体外培养携带志贺毒素基因的噬菌体有关。
Pulsed-field gel electrophoresis (PFGE) of XbaI-digested DNA fragments of enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains showed disappearance of a 70- or 80-kb fragment in their patterns associated with loss of Shiga toxin genes during maintenance or subcultivation. Hybridization experiments with a DNA probe complementary to Shiga toxin sequences revealed that the Shiga toxin genes in the parental strain were located on fragments the same size as the lost fragments from the toxin-negative derivatives, The evidence indicates that PFGE pattern of EHEC O157:H7 may change due to loss of Shiga toxin genes, which is likely to be associated with curing of Shiga toxin gene carrying phages in vitro.