Effect of tunicamycin on rotavirus assembly and infectivity
Effect of tunicamycin on rotavirus assembly and infectivity
复制标题
衣霉素对轮状病毒组装和感染性的影响
作者：
M. Sabara;L. Babiuk;J. Gilchrist;V. Misra
分析了在有或无衣霉素存在的条件下培养的牛轮状病毒的感染性病毒产量、完整颗粒与不完全颗粒的比例以及多肽组成。氯化铯梯度分析表明，衣霉素浓度为1微克/毫升时，病毒产量减少4个对数，并完全阻止[~3H]尿苷掺入完整的轮状病毒颗粒中。随着完整颗粒的减少，三种轮状病毒多肽在聚丙烯酰胺凝胶上的相对位置分别从41900K(41.9K)、29.3K和16.1K移动到35.5K、22.7K和15.5K下的迁移。限制性蛋白水解法表明，小分子多肽与大分子多肽具有相同的组成多肽，这表明它们代表了未糖基化的等价物。这些结果表明，干扰糖基化阻碍了牛轮状病毒外壳蛋白的正确组装。
Bovine rotavirus grown in the presence or absence of tunicamycin was analyzed with respect to yield of infectious virus, the ratio of complete to incomplete particles, and polypeptide composition. Tunicamycin at a concentration of 1 microgram/ml reduced virus yields by 4 logs and completely prevented the incorporation of [3H]uridine into complete rotavirus particles, as determined by cesium chloride gradient analysis. Concomitant with a reduction in complete particles, three rotavirus polypeptides shifted in their relative position on polyacrylamide gels from 41,900-molecular-weight position (41.9K), 29.3K, and 16.1K to migrate at 35.5K, 22.7K, and 15.5K, respectively. Limited proteolysis indicated that the lower-molecular-weight polypeptides possessed the same constituent peptides as the larger polypeptides, suggesting that they represented the unglycosylated equivalents. These results suggest that interference with glycosylation prevents proper assembly of the outer coat proteins in bovine rotavirus.