Bacteroides coprosuis sp. nov., isolated from swine-manure storage pits.
Bacteroides coprosuis sp. nov., isolated from swine-manure storage pits.
复制标题
猪粪拟杆菌。
DOI：
10.1099/ijs.0.63869-0
复制
发表时间：
2005
影响因子：
2.8
通讯作者：
P. Lawson
中科院分区：
文献类型：
--
作者：
T. Whitehead;M. Cotta;M. Collins;E. Falsen;P. Lawson
两个革兰氏阴性，厌氧，非孢子形成，杆状生物体分离自猪粪存储坑。根据形态学和生物化学标准，这些菌株被初步鉴定为属于拟杆菌属，但它们似乎不对应于该属的任何公认种。比较16 S rRNA基因测序研究表明，这些菌株彼此密切相关，并证实它们属于拟杆菌属，但与参考拟杆菌属物种的序列差异值> 10%，表明来自粪肥的生物体代表一个新物种。根据生化指标和分子遗传学证据，从粪便中分离到的未知菌株被认为是拟杆菌属的一个新种，即粪类拟杆菌（Bacteroides coprosuis sp. nov.），模式菌株为PC 139 T（=CCUG 50528 T =NRRL B-41113 T）。
Two Gram-negative, anaerobic, non-spore-forming, rod-shaped organisms were isolated from a swine-manure storage pit. Based on morphological and biochemical criteria, the strains were tentatively identified as belonging to the genus Bacteroides but they did not appear to correspond to any recognized species of the genus. Comparative 16S rRNA gene sequencing studies showed that the strains were related closely to each other and confirmed their placement in the genus Bacteroides, but sequence divergence values of >10 % from reference Bacteroides species demonstrated that the organisms from manure represent a novel species. Based on biochemical criteria and molecular genetic evidence, it is proposed that the unknown isolates from manure be assigned to a novel species of the genus Bacteroides, as Bacteroides coprosuis sp. nov. The type strain is PC139T (=CCUG 50528T=NRRL B-41113T).