Chloroplast DNA haplotype variation and population differentiation in Sorbus aucuparia L. (Rosaceae: Maloideae)
Chloroplast DNA haplotype variation and population differentiation in Sorbus aucuparia L. (Rosaceae: Maloideae)
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DOI：
10.1046/j.1365-294x.2000.00977.x
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发表时间：
2000-08-01
影响因子：
4.9
通讯作者：
Jacquemart, AL
中科院分区：
文献类型：
--
作者：
Raspé, O;Saumitou-Laprade, P;Jacquemart, AL
研究了桃叶花楸（Sorbus aucuparia L.）叶绿体DNA的种内变异，一种虫媒的，中期或早期连续生长的树，结肉质果实。叶绿体基因组的8个PCR扩增片段进行了筛选的限制性片段长度多态性，使用一个或两个4 bp切割限制性内切酶。在两个地理尺度上研究了cpDNA变异：（1）在法国和比利时的四个地区之间;（2）在比利时地区内。共分析了来自6个种群的150个个体。在6个cpDNA片段中检测到14个多态性。所有的多态性可能导致插入或缺失，并允许12个单倍型的鉴定。基于单倍型频率计算的遗传分化水平在所考虑的两个地理尺度上相似（地区之间G（STc）= 0.286，比利时地区内种群之间G（STc）= 0.259）。这些值是远远低于在9个以前研究的温带树种，这是所有风授粉，后期演替产生干果的物种。这些结果可能主要是由于S.桃金娘为了深入了解花粉和种子对基因流的相对贡献，还将G（STc）与先前基于等位酶数据获得的G（ST）估计值进行了比较。
Intra-specific chloroplast DNA (cpDNA) variation was studied in Sorbus aucuparia L., an entomophilous, mid-or early successional tree producing fleshy fruits. Eight PCR-amplified fragments of the chloroplast genome were screened for restriction fragment length polymorphisms, using one or two 4 bp-cutter restriction endonucleases. cpDNA variation was investigated on two geographical scales: (1) among four regions in France and Belgium; and (2) within the Belgian region. A total of 150 individuals from six populations were analysed. Fourteen polymorphisms were detected in six of the cpDNA fragments. All polymorphisms probably resulted from insertions or deletions, and allowed the identification of 12 haplotypes. The level of genetic differentiation computed on the basis of haplotype frequencies was similar on the two geographical scales considered (G(STc) = 0.286 among regions, G(STc) = 0.259 among populations within the Belgian region). These values are much lower than those obtained in nine previously studied temperate tree species, which are all wind-pollinated, late-successional species producing dry fruits. These results might primarily be accounted for by the contrasting life history traits of S. aucuparia. In order to obtain insights into the relative contribution of pollen and seeds to gene flow G(STc) was also compared with previously obtained G(ST) estimates based on allozyme data.