Chronic Al2O3-nanoparticle exposure causes neurotoxic effects on locomotion behaviors by inducing severe ROS production and disruption of ROS defense mechanisms in nematode Caenorhabditis elegans
Chronic Al2O3-nanoparticle exposure causes neurotoxic effects on locomotion behaviors by inducing severe ROS production and disruption of ROS defense mechanisms in nematode Caenorhabditis elegans
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慢性 Al2O3 纳米颗粒暴露通过诱导严重的 ROS 产生和破坏线虫的 ROS 防御机制，对运动行为产生神经毒性作用
DOI：
10.1016/j.jhazmat.2012.03.083
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发表时间：
2012-06-15
影响因子：
13.6
通讯作者：
Wang, Dayong
中科院分区：
文献类型：
--
作者：
Li, Yinxia;Yu, Shunhui;Wang, Dayong
到目前为止，关于慢性纳米毒性机制的知识在很大程度上仍然很少。在本研究中，研究了长期(10天)的氧化铝纳米颗粒(NPs)对秀丽线虫运动行为的影响。暴露于0.01-23.1 mg/L的Al_2O_3-NPs可引起线虫的运动行为下降、严重的应激反应和严重的氧化应激；然而，只有在暴露于23.1 mg/L的原生Al_2O_3的线虫中才能检测到这些影响。Al_2O_3-NPs诱导线虫体内氧化应激的形成既是由于ROS产生的增加，也是由于ROS防御机制的抑制。与块状Al_2O_3相比，暴露于Al_2O_3-NPs的线虫体内的ROS增加，SOD活性降低，编码Mn-SOD的基因(sod-2和sod-3)的表达减少。此外，使用抗氧化剂或过量表达SOD-3不仅可以抑制氧化应激，还可以防止暴露于氧化铝-纳米颗粒对运动行为的不利影响。因此，长期暴露于Al_2O_3-NPs可能通过诱导ROS产生和破坏ROS防御机制对运动行为产生不利影响。此外，突变体sod-2和sod-3比野生型更容易受到三氧化二铝纳米颗粒的慢性神经毒性抑制。(C)2012爱思唯尔B.V.保留所有权利。
To date, knowledge on mechanisms regarding the chronic nanotoxicity is still largely minimal. In the present study, the effect of chronic (10-day) Al2O3-nanoparticles (NPs) toxicity on locomotion behavior was investigated in the nematode Caenorhabditis elegans. Exposure to 0.01-23.1 mg/L of Al2O3-NPs induced a decrease in locomotion behavior, a severe stress response, and a severe oxidative stress; however, these effects were only detected in nematodes exposed to 23.1 mg/L of bulk Al2O3. Formation of significant oxidative stress in nematodes exposed to Al2O3-NPs was due to both the increase in ROS production and the suppression of ROS defense mechanisms. More pronounced increases in ROS, decreases in SOD activity, and decrease in expression of genes encoding Mn-SODs (sod-2 and sod-3) were detected in nematodes exposed to Al2O3-NPs compared with bulk Al2O3. Moreover, treatment with antioxidants or SOD-3 overexpression not only suppressed oxidative stress but also prevented adverse effects on locomotion behaviors from Al2O3-NPs exposure. Thus, chronic exposure to Al2O3-NPs may have adverse effects on locomotion behaviors by both induction of ROS production and disruption of ROS defense mechanisms. Furthermore, sod-2 and sod-3 mutants were more susceptible than the wild-type to chronic Al2O3-NPs-induced neurotoxicity inhibition. (C) 2012 Elsevier B.V. All rights reserved.