Preservation of foraminifera for DNA extraction and PCR amplification
Preservation of foraminifera for DNA extraction and PCR amplification
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DOI：
10.2113/gsjfr.26.3.264
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发表时间：
1996-07-01
影响因子：
1.1
通讯作者：
Pawlowski, J
中科院分区：
文献类型：
--
作者：
Holzmann, M;Pawlowski, J
我们研究了在不同条件下(风干、冷冻、化学固定)储存有孔虫对有孔虫DNA聚合酶链式反应(PCR)扩增的影响。冷冻样品和风干样品效果最好。风干样品在室温下保存11周即可成功扩增，保存2年和3年的有孔虫也可获得阳性结果，但扩增产物大小限制在500个碱基对。乙醇和甲醛固定样品扩增的DNA片段大小也是有限的。干燥标本经扫描电子显微镜(SEM)检查后，成功地扩增出DNA，这为比较研究有孔虫的分子和形态变异开辟了新的视角。
We have examined the effect of storing foraminifera under various conditions (air-drying, freezing, chemical fixation) on the polymerase chain reaction (PCR) amplification of foraminiferal DNA. The best results were obtained with frozen and air-dried samples. PCR amplification was successful with air-dried samples stored at room temperature for up to 11 weeks, Positive results were also obtained with foraminifera stored for 2 and 3 years, although the size of amplification products was limited to 500 bp. The size of DNA fragments amplified from ethanol and formaldehyde fixed samples was also limited. Successful amplification of DNA was achieved from dried specimens following their examination by scanning electron microscopy (SEM), which opens new perspectives for comparative studies of molecular and morphologic variations in foraminifera.