Measurements on the internal structure of freeze-fractured cardiac plasma membrane.
Measurements on the internal structure of freeze-fractured cardiac plasma membrane.
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冷冻断裂心脏质膜内部结构的测量。
DOI：
10.1152/ajpheart.1985.248.3.h297
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Page,E
中科院分区：
文献类型：
--
作者：
Kordylewski,L;Karrison,T;Page,E
我们描述了一个定量分析的内部结构的心脏质膜冷冻断裂在原位，包括P-面颗粒密度（λ），P-面颗粒直径（d），颗粒所占的断裂面面积的百分比（Ap），和颗粒的空间分布（随机，集群，或有序）。这种分析已被应用到7只羊的心脏比较质膜的内部结构在心室和心房的心肌细胞和浦肯野束相同的心脏，也对肌细胞的青蛙，鸡，兔，和大鼠心室（比较内部质膜结构不同的脊椎动物类）。对戊二醛固定和冷冻保护的常规冷冻骨折组织进行测量。我们发现，在相同的绵羊心脏，心室质膜的λ和Ap显着超过心房质膜和心房P-面粒子的分布更集群比心室。d和Ap的青蛙心室质膜P-面显着超过其他一些脊椎动物的值。
We describe a quantitative analysis of the internal structure of cardiac plasma membranes freeze fractured in situ, including the P-face particle density (lambda), the P-face particle diameter (d), the percent of fracture face area occupied by particles (Ap), and the spatial distribution of particles (random, clustered, or ordered). This analysis has been applied to seven sheep hearts to compare the plasmalemmal internal structures in ventricular and atrial myocytes and Purkinje strands of the same hearts and also to myocytes of frog, chicken, rabbit, and rat ventricles (to compare internal plasmalemmal structure of different vertebrate classes). Measurements were made on tissues conventionally prepared for freeze fracture by glutaraldehyde fixation and cryoprotection. We found that, in the same sheep hearts, lambda and Ap for ventricular plasmalemma significantly exceeded those for atrial plasmalemma and that the distribution of atrial P-face particles was more clustered than that for ventricle. d and Ap for frog ventricular plasmalemmal P-face significantly exceeded values for some of the other vertebrates.