Induction of cytopathic effect and cytokines in coxsackievirus B3-infected murine astrocytes.
Induction of cytopathic effect and cytokines in coxsackievirus B3-infected murine astrocytes.
复制标题
柯萨奇病毒 B3 感染的鼠星形胶质细胞细胞病变效应和细胞因子的诱导
DOI：
10.1186/1743-422x-10-157
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发表时间：
2013-05-21
期刊：
影响因子：
4.8
通讯作者：
Li K
中科院分区：
文献类型：
--
作者：
Zeng J;Wang G;Li W;Zhang D;Chen X;Xin G;Jiang Z;Li K
柯萨奇病毒是一种常见的儿童感染病毒，偶尔会引起新生儿严重的脑膜炎和/或脑炎。中枢神经系统病理学背后的潜在机制（S）是不明确defined.MethodsIt假设，星形胶质细胞可能参与炎症反应引起的CVB 3感染。在这里，我们讨论了这一假设的背景下，CVB 3感染和相关的炎症反应在原代小鼠astrocytes.ResultsThe结果表明，柯萨奇病毒受体（CAR）均匀分布在星形胶质细胞，CVB 3可以感染和复制的星形胶质细胞，释放感染性病毒颗粒。CVB 3可诱导星形胶质细胞产生致炎细胞因子IL-1β、TNF-α、IL-6和趋化因子CXCL 10。结论CVB 3直接损伤星形胶质细胞和诱导细胞因子可能是病毒性脑膜炎和/或脑炎的一种机制。
BackgroundCoxsackievirus commonly infects children and occasionally causes severe meningitis and/or encephalitis in the newborn. The underlying mechanism(s) behind the central nervous system pathology is poorly defined.MethodsIt is hypothesized that astrocytes may be involved in inflammatory response induced by CVB3 infection. Here we discuss this hypothesis in the context of CVB3 infection and associated inflammatory response in primary mouse astrocytes.ResultsThe results showed that coxsackievirus receptor (CAR) was distributed homogeneously on the astrocytes, and that CVB3 could infect and replicate in astrocytes, with release of infectious virus particles. CVB3 induced cytopathic effect and production of proinflammatory cytokines IL-1β, TNF-α, IL-6, and chemokine CXCL10 from astrocytes.ConclusionThese data suggest that direct astrocyte damage and cytokines induction could be a mechanism of virus-induced meningitis and/or encephalitis.