Identification of Wnt Genes Expressed in Neural Progenitor Zones during Zebrafish Brain Development.
Identification of Wnt Genes Expressed in Neural Progenitor Zones during Zebrafish Brain Development.
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DOI：
10.1371/journal.pone.0145810
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Dorsky RI
中科院分区：
文献类型：
--
作者：
Duncan RN;Panahi S;Piotrowski T;Dorsky RI
Wnt 信号传导调节脊椎动物中枢神经系统 (CNS) 发育的多个方面，包括神经发生。然而，脊椎动物基因组最多可包含 25 个 Wnt 基因，其功能特征尚不清楚，部分原因是其表达冗余。为了确定候选 Wnt 基因作为特定大脑祖区通路活动的候选介导者，我们在斑马鱼发育过程中的三个不同阶段进行了全面的表达分析。 21 个 Wnt 基因的反义 RNA 探针是从现有的和新合成的 cDNA 克隆中产生的，并用于整个胚胎和解剖大脑的原位杂交。与其他物种一样，我们发现胚胎斑马鱼中枢神经系统中的 Wnt 表达模式很复杂并且通常是多余的。我们观察到端脑、背侧间脑、下丘脑、中脑、中脑-后脑边界、小脑和视网膜的祖区都表达多个Wnt基因。我们的数据确定了 12 种特定配体，现在可以使用功能丧失方法对其进行测试。
Wnt signaling regulates multiple aspects of vertebrate central nervous system (CNS) development, including neurogenesis. However, vertebrate genomes can contain up to 25 Wnt genes, the functions of which are poorly characterized partly due to redundancy in their expression. To identify candidate Wnt genes as candidate mediators of pathway activity in specific brain progenitor zones, we have performed a comprehensive expression analysis at three different stages during zebrafish development. Antisense RNA probes for 21 Wnt genes were generated from existing and newly synthesized cDNA clones and used for in situ hybridization on whole embryos and dissected brains. As in other species, we found that Wnt expression patterns in the embryonic zebrafish CNS are complex and often redundant. We observed that progenitor zones in the telencephalon, dorsal diencephalon, hypothalamus, midbrain, midbrain-hindbrain boundary, cerebellum and retina all express multiple Wnt genes. Our data identify 12 specific ligands that can now be tested using loss-of-function approaches.