Molecular evidence for mother-to-child transmission of Kaposi sarcoma-associated herpesvirus in Uganda and K1 gene evolution within the host
Molecular evidence for mother-to-child transmission of Kaposi sarcoma-associated herpesvirus in Uganda and K1 gene evolution within the host
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DOI：
10.1086/503052
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发表时间：
2006-05-01
影响因子：
6.4
通讯作者：
Whitby, D
中科院分区：
文献类型：
--
作者：
Mbulaiteye, S;Marshall, V;Whitby, D
背景卡波西肉瘤（KS）相关疱疹病毒（KSHV）的流行病学研究表明，KSHV血清阳性的母亲是儿童感染KSHV的危险因素。我们确定了一致的聚合酶链反应阳性的乌干达母子对KSHV K1序列，以确定他们是否共享相同的病毒株。我们还检测了从同一受试者的唾液和血沉棕黄层样本中扩增的序列，以研究潜在的受试者内序列差异。我们从10对母子中的6对中获得了K1序列。在1对中，母亲和孩子之间的亚型不同。另外2对中的母亲和孩子具有相同的亚型，但序列不同。2对母子间KSHV基因同源性为100%。最后一对显示了母亲和孩子之间病毒株一致性的证据，但也显示了孩子体内病毒序列进化的证据。在26名研究受试者中，19名未显示受试者内K1序列变异的证据，但在7名受试者中，所有受试者均为儿童，氨基酸变异为1%-4%。我们的研究结果是一致的KSHV传播从孕产妇和非孕产妇来源的KS流行地区。我们的研究结果也为KSHV感染儿童中K1基因的持续进化提供了证据。
Background. Epidemiological studies of Kaposi sarcoma (KS)-related herpesvirus ( KSHV) indicate that having a KSHV-seropositive mother is a risk factor for KSHV infection in children.Methods. We determined the KSHV K1 sequences in concordantly polymerase chain reaction-positive Ugandan mother-child pairs, to ascertain whether they shared the same viral strain. We also examined sequences amplified from saliva and buffy coat samples from the same subjects, to investigate potential intrasubject sequence differences.Results. We obtained K1 sequences from 6 of 10 mother-child pairs. In 1 pair, the subtypes differed between mother and child. The mother and child in 2 other pairs shared the same subtype, but the sequences differed. The mother and child in 2 pairs shared KSHV strains with exact (100%) nucleotide homology. The last pair showed evidence of viral strain concordance between mother and child but also showed evidence of evolution of the viral sequence within the child. Of 26 study subjects, 19 showed no evidence of intrasubject K1 sequence variability, but, in 7 subjects, all of whom were children, amino acid variation of 1%-4% was observed.Conclusions. Our findings are consistent with KSHV transmission from maternal and nonmaternal sources in KS-endemic regions. Our results also provide evidence for ongoing evolution of the K1 gene in KSHV- infected children.