Antioxidant status, lipid peroxidation and nitric oxide end products in patients of type 2 diabetes mellitus with nephropathy
Antioxidant status, lipid peroxidation and nitric oxide end products in patients of type 2 diabetes mellitus with nephropathy
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DOI：
10.1016/s0009-9120(03)00094-8
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发表时间：
2003-10-01
影响因子：
2.8
通讯作者：
Prabhu, KM
中科院分区：
文献类型：
--
作者：
Bhatia, S;Shukla, R;Prabhu, KM
目的：氧化应激被认为是糖尿病（DM）及其并发症（包括肾病）之间的统一联系。本研究的目的是评估亚裔印度 2 型糖尿病肾病患者的氧化应激状态。 设计和方法：在对照、无肾病的 2 型糖尿病患者（第 1 组）和患有肾病的 2 型糖尿病患者中评估了丙二醛 (MDA) 和一氧化氮终产物（亚硝酸盐和硝酸盐）的血清水平、红细胞超氧化物歧化酶 (SOD) 活性、过氧化氢酶 (CAT) 和还原型谷胱甘肽 (GSH) 含量。肾病（第 2 组）。结果：与对照组相比，两组糖尿病患者的血清 MDA 浓度均显着升高（P < 0.05），其中第 2 组的值显着高于第 1 组（p < 0.05）。与对照组相比，糖尿病患者的血清亚硝酸盐水平显着升高（p < 0.001），但第 1 组和第 2 组之间没有发现显着差异。此外，第 2 组的血清硝酸盐以及亚硝酸盐 + 硝酸盐水平显着高于对照组（p < 0.05）。与对照组相比，两组的红细胞 SOD 和 CAT 活性均显着降低 (p < 0.001)，其中第 2 组的过氧化氢酶活性显着低于第 1 组 (p < 0.05)。与对照组 (p < 0.05) 和第 1 组 (p < 0.05) 相比，第 2 组红细胞 GSH 含量显着降低。结论：本研究结果表明 2 型 DM 中氧化应激增加且抗氧化防御受损。这些紊乱在患有肾病的 2 型糖尿病患者中更为严重。 (C) 2003 年加拿大临床化学家协会。版权所有。
Objectives: Oxidative stress is considered to be a unifying link between diabetes mellitus (DM) and its complications including nephropathy. The aim of the present study was to evaluate oxidative stress status in Asian Indian patients of type 2 DM with nephropathy.Design and Methods: Serum levels of malondialdehyde (MDA) and nitric oxide end products (nitrite and nitrate), activities of erythrocyte superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH) content were estimated in controls, patients of type 2 DM without nephropathy (group 1) and with nephropathy (group 2).Results: Serum MDA concentration was significantly high in both the groups of diabetic patients as compared to controls, (P < 0.05), with group 2 having a significantly higher value than group 1 (p < 0.05). Significantly elevated serum nitrite levels were found in diabetic patients as compared to controls (p < 0.001), however, no significant difference was found between group 1 and group 2. Moreover, serum nitrate as well as nitrite + nitrate levels were significantly higher in group 2 as compared to controls (p < 0.05). Activity of erythrocyte SOD and CAT was significantly reduced in both groups as compared to controls (p < 0.001) with catalase activity in group 2 being significantly lower than group 1 (p < 0.05). Erythrocyte GSH content was significantly lower in group 2 as compared to controls (p < 0.05) and group 1 (p < 0.05).Conclusions: Results of the present study indicate that oxidative stress is increased and antioxidant defenses are compromised in type 2 DM. These derangements are of a higher magnitude in patients of type 2 DM with nephropathy. (C) 2003 The Canadian Society of Clinical Chemists. All rights reserved.