Eimeria tenella infection perturbs the chicken gut microbiota from the onset of oocyst shedding
Eimeria tenella infection perturbs the chicken gut microbiota from the onset of oocyst shedding
复制标题
艾美耳球虫感染从卵囊脱落开始就扰乱鸡肠道微生物群
DOI：
10.1016/j.vetpar.2018.06.005
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发表时间：
2018-07-15
影响因子：
2.6
通讯作者：
Liu, Xianyong
中科院分区：
文献类型：
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作者：
Huang, Guangping;Tang, Xiaoli;Liu, Xianyong
球虫病对家禽业构成严重威胁，在全世界范围内造成重大经济损失。不涉及化疗药物且不会导致抗生素耐药性的球虫病替代疗法的有效开发依赖于对宿主肠道微生物群和肠道球虫之间相互作用的更清晰的了解。在这里，我们建立了鸡艾美耳球虫感染模型，随后使用 16S rRNA 基因测序监测整体肠道微生物组的变化。我们发现受感染鸡的肠道（即粪便）微生物群与未受感染的幼稚动物不同。随着时间的推移，由于柔嫩肠球菌感染，包括乳酸杆菌和粪杆菌在内的非病原菌水平下降，而包括梭状芽胞杆菌、赖氨酸杆菌和埃希氏菌在内的病原菌水平则随着时间的推移而增加。在 Arbor Acres 肉鸡和 White Leghorn 鸡中观察到粪便微生物群的类似动态变化，表明微生物群的扰动是由柔嫩艾美耳球虫感染直接引起的。我们的研究结果可用于进一步阐明球虫感染对宿主健康造成的严重损害，从而开发出新的有效的球虫病治疗方案。
Coccidiosis is a serious threat to the poultry industry, resulting in substantial economic losses worldwide. The effective development of alternative treatments for coccidiosis that does not involve chemotherapy drugs and does not result in antibiotic resistance relies on gaining a clearer understanding of the interaction between host intestinal microbiota and enteric coccidia. Here, we established an Eimeria tenella infection model in chickens and subsequently monitored the changes in the overall intestinal microbiome using 16S rRNA gene sequencing. We found that the gut (i.e. fecal) microbiota of infected chicken differed from that of uninfected naive animals. Levels of non-pathogenic bacteria, including Lactobacillus and Faecalibacterium declined, whereas those of pathogenic bacteria, including Clostridium, Lysinibacillus, and Escherichia, increased over time in response to E. tenella infection. Similar dynamic changes of the fecal microbiota were observed in both Arbor Acres broilers and White Leghorn chickens, indicating that the perturbation of the microbiota was directly induced by E. tenella infection. Our findings could be used to further elucidate the serious damage to host health caused by coccidia infection, leading to the development of new effective treatment options for coccidiosis.