Human U2 and U1 RNA genes use similar transcription signals.
Human U2 and U1 RNA genes use similar transcription signals.
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人类 U2 和 U1 RNA 基因使用相似的转录信号。
DOI：
10.1002/j.1460-2075.1984.tb02293.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Dahlberg,JE
中科院分区：
文献类型：
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作者：
Westin,G;Lund,E;Murphy,JT;Pettersson,U;Dahlberg,JE
我们通过将克隆的U2/6 RNA基因注射到非洲爪蟾卵母细胞细胞核中，分析了人类U2 RNA转录的需求。两种形式的人类U2 RNA积累，一个主要的物种对应于成熟大小的U2 RNA，一个次要的物种对应于一个3'延伸的前体。这种RNA聚合酶II转录只需要258 bp和94 bp的5‘和3’侧区序列。高效的U2 RNA合成依赖于位于-258和-198位置之间的启动子元件。该区域包含一个12bp的直接重复序列，与人类U1 RNA基因的上游启动子元件非常相似。-258和-198之间的序列也赋予U2 RNA模板与共注射的U1 RNA模板完成snRNA基因特异性转录因子的能力。U2 RNA的转录从含有活性RNA聚合酶III转录单元的模板上减少，可能是由于DNA的松弛或隔离。在体外，U2 RNA基因的转录与U1 RNA基因的转录一样，是从体内合成RNA的5'端对应的点上游开始的。图片
We have analyzed the requirements for human U2 RNA transcription by injection of cloned U2/6 RNA genes into nuclei of Xenopus laevis oocytes. Two forms of human U2 RNAs accumulate, a major species corresponding to mature-sized U2 RNA and a minor species corresponding to a 3'-extended precursor. This RNA polymerase II transcription requires only 258 and 94 bp of 5'- and 3'-flanking region sequences, respectively. Efficient U2 RNA synthesis depends on a promoter element located between positions -258 and -198. This region contains a 12-bp direct repeat which strongly resembles a comparable upstream promoter element of the human U1 RNA genes. Sequences between -258 and -198 also confer on the U2 RNA template the ability to complete with co-injected U1 RNA templates for a snRNA gene-specific transcription factor(s). Transcription of U2 RNA is reduced off templates containing an active RNA polymerase III transcription unit, presumably because of relaxation or sequestration of the DNA. In vitro transcription of the U2 RNA gene, like that of the U1 RNA gene, is initiated upstream of the point corresponding to the 5' end of in vivo synthesized RNA. Images