Antigen retrieval techniques: Current perspectives
Antigen retrieval techniques: Current perspectives
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DOI：
10.1177/002215540104900801
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发表时间：
2001-08-01
影响因子：
3.2
通讯作者：
Taylor, CR
中科院分区：
文献类型：
--
作者：
Shi, SR;Cote, RJ;Taylor, CR
抗原修复(AR)技术的发展是一种简单的方法，将档案石蜡包埋的组织切片在水中煮沸以增强免疫组织化学(IHC)的信号，这是开创性努力的成果，其指导思想是使IHC适用于常规的福尔马林固定的石蜡包埋组织，以便在研究和临床病理学中广泛应用。在数千篇文章和许多评论的基础上，最近出版了一本书，总结了AR技术的实践和进一步发展的基本原则。本文重点介绍了AR技术的定义、应用、技术原理和进一步研究等几个关键问题。特别是，为了充分提取蛋白质以外的核酸，还进一步应用了热诱导提取方法，并在试验电池方法方面对基于AR技术的常规IHC进行了标准化。此外，了解AR技术的机理可能有助于分子形态的发展。
Development of the antigen retrieval (AR) technique, a simple method of boiling archival paraffin-embedded tissue sections in water to enhance the signal of immunohistochemistry (IHC), was the fruit of pioneering efforts guided by the philosophy of rendering IHC applicable to routine formalin-fixed, paraffin-embedded tissues for wide application of IHC in research and clinical pathology. On the basis of thousands of articles and many reviews, a book has recently been published that summarizes basic principles for practice and further development of the AR technique. Major topics with respect to several critical issues, such as the definition, application, technical principles, and further studies of the AR technique, are highlighted in this article. In particular, a further application of the heat-induced retrieval approach for sufficient extraction of nucleic acids in addition to proteins, and standardization of routine IHC based on the AR technique in terms of a test battery approach, are also addressed. Furthermore, understanding the mechanism of the AR technique may shed light on facilitating the development of molecular morphology.