Awake and behaving mouse fMRI during Go/No-Go task
Awake and behaving mouse fMRI during Go/No-Go task
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DOI：
10.1016/j.neuroimage.2019.01.002
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发表时间：
2019-03-01
期刊：
影响因子：
5.7
通讯作者：
Liang, Zhifeng
中科院分区：
文献类型：
--
作者：
Han, Zhe;Chen, Wenjing;Liang, Zhifeng
功能磁共振成像(FMRI)已被广泛应用于检测诱发状态和静息状态下的功能神经网络。然而，大多数啮齿动物的fMRI研究都是在麻醉下进行的，这极大地限制了它们的应用范围和行为相关性。人们已经努力成像处于清醒状态的啮齿动物，无论是在休息状态，还是在对感觉或光遗传刺激的反应中。然而，在清醒行为的啮齿动物中进行功能磁共振成像还没有实现。在目前的研究中，一种新的fMRI范式被开发出来，用于清醒和行为的小鼠，允许在基于嗅觉的Go/no-Go任务中对小鼠的大脑进行功能成像。利用低温线圈在9.4T下对清醒和行为正常的小鼠进行了运动受限和图像失真的高分辨率功能成像。分布的全脑时空模式被揭示出来，围棋试验和不围棋试验的活动轮廓截然不同。因此，我们已经证明了对清醒小鼠的嗅觉行为进行功能成像的可行性。这种在清醒行为小鼠身上的fMRI范式可能会在整个大脑水平上对潜在行为的神经机制产生新的见解。
Functional magnetic imaging (fMRI) has been widely used to examine the functional neural networks in both the evoked and resting states. However, most fMRI studies in rodents are performed under anesthesia, which greatly limits the scope of their application, and behavioral relevance. Efforts have been made to image rodents in the awake condition, either in the resting state or in response to sensory or optogenetic stimulation. However, fMRI in awake behaving rodents has not yet been achieved. In the current study, a novel fMRI paradigm for awake and behaving mice was developed, allowing functional imaging of the mouse brain in an olfaction-based go/no-go task. High resolution functional imaging with limited motion and image distortion were achieved at 9.4T with a cryogenic coil in awake and behaving mice. Distributed whole-brain spatiotemporal patterns were revealed, with drastically different activity profiles for go versus no-go trials. Therefore, we have demonstrated the feasibility of functional imaging of an olfactory behavior in awake mice. This fMRI paradigm in awake behaving mice could lead to novel insights into neural mechanisms underlying behaviors at a whole-brain level.