DETERMINATION OF THE LIFETIME OF SINGLET OXYGEN IN D2O USING 9,10-ANTHRACENEDIPROPIONIC ACID, A WATER-SOLUBLE PROBE
DETERMINATION OF THE LIFETIME OF SINGLET OXYGEN IN D2O USING 9,10-ANTHRACENEDIPROPIONIC ACID, A WATER-SOLUBLE PROBE
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DOI：
10.1021/ja00537a030
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发表时间：
1980-01-01
影响因子：
15
通讯作者：
SCHAAP, AP
中科院分区：
文献类型：
--
作者：
LINDIG, BA;RODGERS, MAJ;SCHAAP, AP
一种水溶性单线态氧监测仪用于时间分辨激光光解实验。用单线态氧将9,10-蒽二丙酸（ADPA）二钠盐漂白成内过氧化物。这种吸光度的变化被动力学光谱法跟踪。激光激发亚甲基蓝形成单重态氧，ADPA的衰变为一级。测定D2O中ADPA漂白速率常数与ADPA浓度的关系，得到斜率为(8.2 .+-。0.5)同学们。107 L mol-1 s-1为ADPA猝灭单线态氧的双分子速率常数。这条直线的截距是（1.9 .+-）。0.1)同学们。104 s-1，对应于D2O中单线态氧自然衰变的速率常数。.tau…DELTA的结果值。53 .+-。3 .mu。s，证实了先前确定的含有离子表面活性剂胶束的D2O溶液中单线态氧寿命的值。ADPA是一种简便、特异的检测水系统中单线态氧存在和衰变的监测仪。为了进一步证明这一点，用ADPA测定了组氨酸在D2O中单线态氧猝灭的速率常数为（6.1 .+-）。0.5)同学们。107 L mol-1 s-1，这一数值与先前用呋喃DPBF作为单线态氧监测仪在表面活性剂溶液和醇-水混合物中测定的结果很一致。
A H2O-soluble singlet oxygen monitor has been used in time-resolved laser photolysis experiments. The disodium salt of 9,10-anthracenedipropionic acid (ADPA) is bleached to an endoperoxide on reaction with singlet oxygen. This change in absorbance was followed by kinetic spectrometry. Singlet oxygen was formed by laser excitation of methylene blue and the resulting decay of ADPA was 1st order. Measurement of the rate constant for ADPA bleaching in D2O as a function of ADPA concentration yielded a line whose slope, (8.2 .+-. 0.5) .times. 107 L mol-1 s-1, is the bimolecular rate constant for quenching of singlet oxygen by ADPA. The intercept of this line, (1.9 .+-. 0.1) .times. 104 s-1, corresponds to the rate constant for natural decay of singlet oxygen in D2O. The resulting value for .tau..DELTA., 53 .+-. 3 .mu.s, substantiates the previously determined values for the singlet oxygen lifetime in D2O solutions containing ionic surfactant micelles. ADPA shows itself to be a convenient and specific monitor for detecting the presence and decay of singlet oxygen in aqueous systems. To demonstrate this further, the rate constant for singlet oxygen quenching by histidine in D2O has been determined, using ADPA, to be (6.1 .+-. 0.5) .times. 107 L mol-1 s-1, a value which is in good agreement with those previously determined using the furan DPBF as singlet oxygen monitor, both in surfactant solution and in alcohol-water mixtures.