P66 porins are present in both Lyme disease and relapsing fever spirochetes: A comparison of the biophysical properties of P66 porins from six Borrelia species
P66 porins are present in both Lyme disease and relapsing fever spirochetes: A comparison of the biophysical properties of P66 porins from six Borrelia species
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DOI：
10.1016/j.bbamem.2010.02.011
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发表时间：
2010-06-01
影响因子：
3.4
通讯作者：
Benz, Roland
中科院分区：
文献类型：
--
作者：
Barcena-Uribarri, Ivan;Thein, Marcus;Benz, Roland
疏螺旋体属是导致人类两种疾病：莱姆病(LD)和复发热(RF)的原因。LD和RF疏螺旋体都是专性寄生虫，依赖宿主提供的营养物质。这些革兰氏阴性细菌通过外膜吸收营养的第一步是通过充满水的通道，即所谓的孔蛋白来完成的。对不同致病疏螺旋体外膜中的孔蛋白组成的了解有限。到目前为止，在复发热螺旋体中只有一种孔蛋白被描述，而在莱姆病药物中已知存在四种孔蛋白。因此，伯氏疏螺旋体外膜通道p66被认为是一种粘附素，并被广泛研究为孔蛋白。为了研究p66孔蛋白是否在其他引起莱姆病或复发性发热的疏螺旋体中表达并具有类似的孔道形成能力，研究了三个LD种(B.burgdorferi、B.afzelii、B.garinii)和三个RF种(B.duttonii、B.recrentis和B.hermsii)与p66同源的外膜蛋白。对目前已发表的包括杜通氏杆菌、递归杆菌和赫姆斯氏杆菌的RF基因组的搜索表明，它们都含有p66同源物。对6种疏螺旋体的p66同源物进行了纯化，并用黑脂双层法分析了它们的成孔能力和毛孔的生物物理性质。(C)2010爱思唯尔B.V.保留所有权利。
The genus Borrelia is the cause of the two human diseases: Lyme disease (LD) and relapsing fever (RF). Both LD and RF Borrelia species are obligate parasites and are dependent on nutrients provided by their hosts. The first step of nutrient uptake across the outer membrane of these Gram-negative bacteria is accomplished by water-filled channels, so-called porins. The knowledge of the porin composition in the outer membranes of the different pathogenic Borrelia species is limited. Only one porin has been described in relapsing fever spirochetes to date, whereas four porins are known to be present in Lyme disease agents. From these, the Borrelia burgdorferi outer membrane channel P66 is known to act as an adhesin and was well studied as a porin. To investigate if P66 porins are expressed and similarly capable of pore formation in other Borrelia causing Lyme disease or relapsing fever three LD species (B. burgdorferi, B. afzelii, B. garinii) and three RF species (B. duttonii, B. recurrentis and B. hermsii) were investigated for outer membrane proteins homologous to P66. A search in current published RF genomes, comprising the ones of B. duttonii, B. recurrentis and B. hermsii, indicated that they all contained P66 homologues. The P66 homologues of the six Borrelia species were purified to homogeneity and their pore-forming abilities as well as the biophysical properties of the pores were analyzed using the black lipid bilayer assay. (C) 2010 Elsevier B.V. All rights reserved.