Cell-dependent requirement of human immunodeficiency virus type 1 gp41 cytoplasmic tail for Env incorporation into virions
Cell-dependent requirement of human immunodeficiency virus type 1 gp41 cytoplasmic tail for Env incorporation into virions
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DOI：
10.1128/jvi.74.10.4891-4893.2000
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发表时间：
2000-05-01
影响因子：
5.4
通讯作者：
Adachi, A
中科院分区：
文献类型：
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作者：
Akari, H;Fukumori, T;Adachi, A
研究了两种缺失 gp41 胞质尾部的人类免疫缺陷病毒 1 型 (HIV-1) 突变体的淋巴细胞 H9 和 M8166 细胞的生长动力学。虽然命名为 CTdel-44 和 CTdel-144 的突变病毒能够在 M8166 细胞中生长，但它们无法在 H9 细胞中生长。转染和单轮感染性测定表明它们在 H9 细胞中病毒复制的早期阶段存在缺陷。对突变病毒体的分析表明，H9 细胞中 Env gp120 的掺入量大幅减少（与野生型病毒体的掺入量相比），但 M8166 细胞中的掺入量正常。这些结果表明，gp41 的 HIV-1 胞质尾通过影响 Env 掺入病毒粒子，以细胞依赖性方式决定病毒感染性，并表明宿主细胞因子参与 Env 掺入。
Growth kinetics in lymphocytic H9 and M8166 cells of two mutants of human immunodeficiency virus type 1 (HIV-1) with deleted gp41 cytoplasmic tails were examined. While the mutant viruses designated CTdel-44 and CTdel-144 were able to grow in M8166 cells, they were unable to grow in H9 cells. Transfection and single-round infectivity assays demonstrated that they are defective in the early phase of viral replication in H9 cells. Analysis of the mutant virions revealed drastically reduced incorporation of Env gp120 (compared with the incorporation of wild-type virions) in H9 cells but normal incorporation in M8166 cells. These results indicate that the HIV-1 cytoplasmic tail of gp41 determines virus infectivity in a cell-dependent manner by affecting incorporation of Env into virions and suggest the involvement of a host cell factor(s) in the Env incorporation.