An extended feeding history with a stearidonic acid enriched diet from parr to smolt increases n-3 long-chain polyunsaturated fatty acids biosynthesis in white muscle and liver of Atlantic salmon (Salmo salar L.)
An extended feeding history with a stearidonic acid enriched diet from parr to smolt increases n-3 long-chain polyunsaturated fatty acids biosynthesis in white muscle and liver of Atlantic salmon (Salmo salar L.)
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DOI：
10.1016/j.aquaculture.2011.09.014
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发表时间：
2011-12-21
期刊：
影响因子：
4.5
通讯作者：
Carter, Chris G.
中科院分区：
文献类型：
--
作者：
Codabaccus, Basseer M.;Bridle, Andrew R.;Carter, Chris G.
植物油是全球公认的水产饲料中鱼油的替代品。VO中缺乏n-3长链(>；=C-20)多不饱和脂肪酸(n-3LC-PUFA)是一个主要制约因素。玉米油(EO)富含硬脂酸(SDA)，具有促进内源n-3LC-PUFA生物合成的潜力。我们测试了在淡水和海水中以EO为基础的饲料喂养大西洋鲑鱼是否会增加n-3LC-PUFA的水平，方法是将肝脏和白色肌肉中的脂肪酸(FA)谱与饲喂以FO和菜籽油(RO)为基础的饲料的鱼进行比较。通过对n-3LC-PUFA生物合成酶基因表达的测定，揭示了n-3LC-PUFA生物合成的内在机制。在从淡水到海水阶段长时间摄食EO饲料后，EO鱼肝脏和白色肌肉中的n-3LC-PUFA水平均高于RO鱼。然而，FO FISH的n-3LC-PUFA水平在被检测组织中最高。Delta6脱氢酶基因在肝脏和白色肌肉中的表达仅在RO鱼中上调，在海水中的表达降低，肝脏FA伸长酶基因的表达受食用油和环境相互作用的调节。这项研究表明，通过增加n-3LC-PUFA前体SDA的供应，使用SDA丰富的饲料从Parr到Smolt饲养大西洋鲑鱼可以增加肝脏和白色肌肉中n-3LC-PUFA的生物合成。海水鱼肝脏Delta 5去饱和酶基因表达下调可能解释了n-3LC-PUFA生物合成的环境差异。(C)2011爱思唯尔B.V.保留所有权利。
Vegetable oils (VO) are globally accepted alternatives for fish oil (FO) in aquafeeds. The lack of n-3 long-chain (>= C-20) polyunsaturated fatty acids (n-3 LC-PUFA) in VO is a major constraint. Echium oil (EO), rich in stearidonic acid (SDA), has the potential to increase endogeneous n-3 LC-PUFA biosynthesis. We tested whether feeding Atlantic salmon an EO-based diet in both freshwater and seawater would increase n-3 LC-PUFA levels by comparing the fatty acid (FA) profiles in liver and white muscle to fish fed FO and rapeseed oil (RO)-based diets. The gene expression of n-3 LC-PUFA biosynthetic enzymes was measured to demonstrate the underlying mechanism of n-3 LC-PUFA biosynthesis. After prolonged feeding with EO diet from freshwater to seawater phases, EO fish had higher n-3 LC-PUFA levels in both liver and white muscle compared to RO fish. However, FO fish had the highest n-3 LC-PUFA levels in examined tissues. Delta 6 Desaturase gene expression in liver and white muscle was up-regulated in RO fish only, liver Delta 5 desaturase gene expression was reduced in seawater and liver FA elongase gene expression was regulated by an interaction between dietary oil and environment. This study showed that feeding Atlantic salmon from parr to smolt using an SDA enriched diet increases n-3 LC-PUFA biosynthesis in liver and white muscle through increased supply of the n-3 LC-PUFA precursor SDA. The down regulation of Delta 5 desaturase gene expression in the liver of seawater fish may explain environmental differences in n-3 LC-PUFA biosynthesis. (C) 2011 Elsevier B. V. All rights reserved.