Optimal algorithm for single-molecule identification with time-correlated single-photon counting
Optimal algorithm for single-molecule identification with time-correlated single-photon counting
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DOI：
10.1021/jp002358n
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发表时间：
2001-01-11
影响因子：
2.9
通讯作者：
Sauer, M
中科院分区：
文献类型：
--
作者：
Enderlein, J;Sauer, M
提出了一种根据荧光的时间衰减特性识别单分子的模式匹配程序，并将其应用于测量三种不同染料的单分子数据。基于最大似然原理，如果确切地知道不同分子种类的荧光时间衰减，则该过程是最好的识别算法。所提出的算法在数值上简单且快速，因此特别适合可能的在线应用。推导出算法误识别误差的精确理论结果，并将其与实验数据确定的误差进行比较。
A pattern-matching procedure for identifying single molecules according to the temporal decay characteristics of the fluorescence is presented and applied to measured single-molecule data of three different dyes. Based on the maximum likelihood principle, this procedure is the best possible identification algorithm if the fluorescence time decays of the different molecular species are exactly known. The presented algorithm is numerically simple and fast and, thus, especially suitable to possible online applications. Exact theoretical results for;he misidentification errors of the algorithm are derived and compared with the errors determined from the experimental data.