The sceptical optimist: challenges and perspectives for the application of environmental DNA in marine fisheries
The sceptical optimist: challenges and perspectives for the application of environmental DNA in marine fisheries
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DOI：
10.1111/faf.12286
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发表时间：
2018-09-01
影响因子：
6.7
通讯作者：
Nielsen, Einar Eg
中科院分区：
文献类型：
--
作者：
Hansen, Brian Klitgaard;Bekkevold, Dorte;Nielsen, Einar Eg
环境DNA(EDNA)分析的应用已引起海洋生物资源和生物多样性研究人员、顾问和管理者的关注。EDNA分析的明显简单性和成本效益使其极具吸引力，因为可以从水样中揭示物种分布。此外，针对具体物种的分析表明，EDNA浓度与生物量和丰度相关，这表明定量应用估计海洋生态系统中特定生物的丰度和生物量的可能性，例如用于种群评估。然而，从检测生物体的出现到定量估计的过程是漫长和间接的，尤其是因为EDNA的浓度取决于几个影响其在海洋生态系统中的生产、持久性和运输的物理、化学和生物因素。在这里，我们提供了与EDNA分析相关的基本原理的概述，并具有海洋渔业应用的潜力。我们描述了支配EDNA产生、击穿和传输的基本过程，并总结了目前关于这些过程的不确定性。我们描述了在渔业评估中应用的五个主要挑战，在渔业评估中，迫切需要在海洋系统中建立知识，并指出EDNA与现有的海洋渔业监测方法相比存在明显的弱点。我们概述了渔业管理中新出现的令人感兴趣的应用，并指出了可以提高分析效率的最新技术进步。我们建议防止夸大EDNA分析目前在渔业监测中的应用范围，但也认为，通过对优势和局限性的知情见解，EDNA分析可以成为渔业评估和管理的综合工具。
Application of environmental DNA (eDNA) analysis has attracted the attention of researchers, advisors and managers of living marine resources and biodiversity. The apparent simplicity and cost-effectiveness of eDNA analysis make it highly attractive as species distributions can be revealed from water samples. Further, species-specific analyses indicate that eDNA concentrations correlate with biomass and abundance, suggesting the possibility for quantitative applications estimating abundance and biomass of specific organisms in marine ecosystems, such as for stock assessment. However, the path from detecting occurrence of an organism to quantitative estimates is long and indirect, not least as eDNA concentration depends on several physical, chemical and biological factors which influence its production, persistence and transport in marine ecosystems. Here, we provide an overview of basic principles in relation to eDNA analysis with potential for marine fisheries application. We describe fundamental processes governing eDNA generation, breakdown and transport and summarize current uncertainties about these processes. We describe five major challenges in relation to application in fisheries assessment, where there is immediate need for knowledge building in marine systems, and point to apparent weaknesses of eDNA compared to established marine fisheries monitoring methods. We provide an overview of emerging applications of interest to fisheries management and point to recent technological advances, which could improve analysis efficiency. We advise precaution against exaggerating the present scope for application of eDNA analysis in fisheries monitoring, but also argue that with informed insights into strengths and limitations, eDNA analysis can become an integrated tool in fisheries assessment and management.