Quantification of adipose tissue leukocytosis in obesity.
Quantification of adipose tissue leukocytosis in obesity.
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DOI：
10.1007/978-1-62703-523-1_15
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发表时间：
2013
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影响因子：
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通讯作者：
Dixit, Vishwa Deep
中科院分区：
文献类型：
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作者：
Grant, Ryan;Youm, Yun-Hee;Ravussin, Anthony;Dixit, Vishwa Deep
免疫细胞亚群在脂肪组织中的浸润称为“脂肪组织白细胞增多症”，是慢性炎症和肥胖相关共病发展中的关键事件。考虑到肥胖患者脂肪组织中相当大比例的细胞具有造血谱系，不同的脂肪库代表了可以影响代谢功能的未表征的免疫器官。在这里，我们描述的方法来表征和分离白细胞从复杂的脂肪组织微环境，以帮助机械研究，以了解特定的模式识别受体（PRR），如脂肪免疫串扰中的炎性小体的作用。
The infiltration of immune cell subsets in adipose tissue termed ‘adipose tissue leukocytosis’ is a critical event in the development of chronic inflammation and obesity-associated comorbidities. Given that a significant proportion of cells in adipose tissue of obese patients are of hematopoietic lineage, the distinct adipose depots represent an uncharacterized immunological organ that can impact metabolic functions. Here, we describe approaches to characterize and isolate leukocytes from the complex adipose tissue microenvironment to aid mechanistic studies to understand the role of specific pattern recognition receptors (PRRs) such as inflammasomes in adipose-immune crosstalk.