Transplantation and repair: Combined cell implantation and chondroitinase delivery prevents deterioration of bladder function in rats with complete spinal cord injury
Transplantation and repair: Combined cell implantation and chondroitinase delivery prevents deterioration of bladder function in rats with complete spinal cord injury
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DOI：
10.1038/sc.2009.10
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发表时间：
2009-10-01
期刊：
影响因子：
2.2
通讯作者：
Vavrek, R.
中科院分区：
文献类型：
--
作者：
Fouad, K.;Pearse, D. D.;Vavrek, R.
研究设计：附加检查。在这项研究中，我们报告了膀胱功能的变化后，联合治疗，旨在研究轴突再生后，完全脊髓损伤（SCI）大鼠。目的：报告膀胱功能的影响后，管理的联合治疗完全SCI。设置：阿尔伯塔大学，康复医学系，埃德蒙顿，加拿大方法：8只大鼠在完全胸段脊髓损伤后的病变部位接受了基质胶填充的引导通道、嗅鞘神经胶质和软骨素酶ABC中的雪旺细胞。对照组（n = 7）仅接受基质胶。每日进行膀胱检查。膀胱大小，壁厚，肌动蛋白和胶原蛋白III型进行了分析后14 weeks.Results：脊髓损伤后，两组恢复膀胱排尿后3周。然而，2周后，在所有未处理的大鼠和2只处理的大鼠中观察到失禁。对膀胱的尸检显示膀胱增大。较厚的膀胱壁被发现在未经处理的大鼠，这是由高量的胶原蛋白（III型）包围的平滑肌纤维的杂乱束组成的。结论：我们表明，联合治疗可以防止胶原蛋白沉积在膀胱壁，并保持大鼠的能力，有效地排尿。虽然这种改善的机制尚不清楚，但我们的研究表明，目前的联合治疗可以影响膀胱功能，从而扩大其作为SCI广泛修复方法的实用性。Spinal Cord（2009）47，727-732; doi：10.1038/sc.2009.10; 2009年3月3日在线发表
Study design: Additional examination. In this study, we report changes in bladder function after a combined treatment that was designed to study axonal regeneration after complete spinal cord injury (SCI) in rats.Objectives: To report effects on bladder function following the administration of a combined treatment for complete SCI.Setting: University of Alberta, Faculty of Rehabilitation Medicine, Edmonton, CanadaMethods: Eight rats received Schwann cells in Matrigel-filled guidance channels, olfactory ensheathing glia and chondroitinase ABC at the lesion site following complete thoracic SCI. Controls (n = 7) received Matrigel only. Daily bladder examinations were performed. Analysis of bladder size, wall thickness, actin and collagen type III was performed after 14 weeks.Results: Following SCI, both groups regained bladder voiding after 3 weeks. However, 2 weeks later, incontinence was observed in all untreated rats and two treated rats. Post-mortem examination of bladders revealed enlarged bladder sizes. Thicker bladder walls were found in untreated rats, which were composed of disorganized bundles of smooth muscle fibers surrounded by high amounts of collagen (type III).Conclusion: We show that the combined treatment prevents collagen deposition in bladder walls and maintains the rat's ability to void efficiently. Although the mechanism responsible for this improvement is unclear, our study shows that the present combinatory therapy can influence bladder function, thus expanding their utility as a broad reparative approach for SCI. Spinal Cord (2009) 47, 727-732; doi: 10.1038/sc.2009.10; published online 3 March 2009