Visualization and characterization of GB virus-C particles: evidence for a nucleocapsid.
Visualization and characterization of GB virus-C particles: evidence for a nucleocapsid.
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GB 病毒 C 颗粒的可视化和表征：核衣壳的证据。
DOI：
10.1046/j.1365-2893.1999.00003.x
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Stapleton,JT
中科院分区：
文献类型：
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作者：
Xiang,J;Daniels,KJ;Soll,DR;Schmidt,WN;Labrecque,DR;Stapleton,JT
GB病毒C型（GBV-C）是黄病毒科肝炎病毒属的成员。持续性GBV-C感染在人类中很常见，但目前尚不清楚GBV-C是否会导致任何疾病。虽然GBV‐C感染与急性非A型至非E型输血后肝炎有关，但它似乎不会引起慢性肝炎。GBV-C与丙型肝炎病毒（HCV）密切相关，但间接证据表明其在开放阅读框（ORF）的氨基末端不编码核心蛋白。这导致人们猜测GBV‐C没有核衣壳。我们评估了GBV-C的浮力密度，发现非常低密度的颗粒与病毒体一致，中等密度的颗粒与GBV-C感染者的核衣壳一致。此外，电子显微镜显示在包膜颗粒内有明显的核衣壳。尽管这些生物物理数据强烈表明GBV-C利用了核衣壳，但它们并不能表明该颗粒蛋白质含量的来源。为了评估这一点，我们评估了患者血浆与合成寡肽的反应性，该合成寡肽代表预测ORF氨基末端附近的保守区域。在一些个体中检测到特异性抗体，与Feuchtet等人的数据相似，Feuchtet等人在GBV‐C‐感染者中鉴定出针对重组核心蛋白的抗体。这些数据表明GBV-C颗粒含有核衣壳。至少在某些患者中，GBV-C E1蛋白编码区上游的区域似乎表达，该区域可能代表核衣壳的结构蛋白。
GB virus type C (GBV‐C) is a member of the hepacivirus genus within theFlaviviradae. Persistent GBV‐C infection is common in humans, yet it remains unclear if GBV‐C causes any disease. Although GBV‐C infection has been associated with acute non‐A to non‐E post‐transfusion hepatitis, it does not appear to cause chronic hepatitis. GBV‐C is closely related to hepatitis C virus (HCV), but indirect evidence suggests that it does not encode a core protein at the amino terminus of the open reading frame (ORF). This has led to speculation that GBV‐C does not have a nucleocapsid. We evaluated the buoyant density of GBV‐C, and found very low density particles consistent with virions, and intermediate density particles consistent with nucleocapsids in GBV‐C‐infected people. In addition, electron microscopy demonstrated an apparent nucleocapsid within an enveloped particle. Although these biophysical data strongly suggest that GBV‐C utilizes a nucleocapsid, they do not indicate the origin of the protein content of this particle. To assess this, we evaluated patient plasma for reactivity with a synthetic oligopeptide representing a conserved region near the amino terminus of the predicted ORF. Specific antibody was detected in some individuals, similar to data of Feuchtet al.who identified antibody against a recombinant core protein in GBV‐C‐infected people. These data indicate that GBV‐C particles contain nucleocapsids. At least in some patients, the region upstream of the GBV‐C E1 protein coding region appears to be expressed, and this region may represent the structural protein of the nucleocapsid.