Convenient and Efficient Synthesis of a Lanthanide-Coordinated, Diethylene Triamine Pentaacetic Acid Labeled Biopolymer as an Assay for the Cholecystokinin B Receptor.
Convenient and Efficient Synthesis of a Lanthanide-Coordinated, Diethylene Triamine Pentaacetic Acid Labeled Biopolymer as an Assay for the Cholecystokinin B Receptor.
复制标题
方便高效地合成镧系元素配位、二亚乙基三胺五乙酸标记的生物聚合物，用于胆囊收缩素 B 受体的测定。
DOI：
10.1002/app.26910
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Gillies,R
中科院分区：
文献类型：
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作者：
Gao,F;Handl,H;Vagner,J;Hruby,V;Gillies,R
为了建立一种用Eu3+标记的缩胆囊素肽通过二乙基三胺五乙酸螯合连接体检测缩胆囊素B受体的方法，采用固相合成方法，将二氢化二乙基三胺五乙酸前体直接连接到缩胆囊素肽的N端，经反相高效液相色谱分离，获得了满意的产率和纯度。然后通过二乙烯三胺五乙酸双功能剂将镧系元素与肽配位。该方法是大规模合成镧系3+配位、二乙烯三胺五乙酸标记生物聚合物的有效方法。本研究不仅为制备基于镧系元素的肽配体文库提供了一种简单方便的方法，而且还为基于镧系元素的肽受体的高通量筛选提供了一种时间分辨荧光分析系统。本研究合成了5种生物聚合物，并用高分辨率电喷雾电离法对其进行了表征。©2007 Wiley期刊公司高分子学报，2007
To develop an assay for the cholecystokinin B receptor with an Eu3+‐labeled cholecystokinin peptide via a diethylene triamine pentaacetic acid chelating linker, a commercial dianhydride diethylene triamine pentaacetic acid precursor was directly attached to the N‐terminus of cholecystokinin peptides by a solid‐phase synthesis method with a satisfactory yield and purity after reverse‐phase high‐performance liquid chromatography separation. Lanthanide was then coordinated to the peptide via a diethylene triamine pentaacetic acid bifunctional agent. This method is a useful approach to the large‐scale synthesis of lanthanide3+‐coordinated, diethylene triamine pentaacetic acid labeled biopolymers. This research provides not only a simple and convenient method for the preparation of lanthanide‐based peptide ligand libraries but also possible lanthanide‐based high‐throughput screening of peptide receptors with a time‐resolved fluorescence assay system. Five biopolymers were synthesized and characterized with high‐resolution electrospray ionization in this study. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007