Inter- and intra-individual variations in seasonal and daily stabilities of the human gut microbiota in Japanese.
Inter- and intra-individual variations in seasonal and daily stabilities of the human gut microbiota in Japanese.
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DOI：
10.1007/s00203-015-1125-0
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发表时间：
2015-09
影响因子：
2.8
通讯作者：
Kuriki K
中科院分区：
文献类型：
--
作者：
Hisada T;Endoh K;Kuriki K
人类肠道微生物区系、饮食习惯和健康/疾病之间的关系是流行病学和临床研究的主题。然而，粪便样本中细菌群落的时间稳定性和变异性仍不清楚。在这项研究中，从樱花饮食研究中招募了没有疾病的日本中年男性和女性志愿者(n=5)。连续7天在每个季度和每次排便时采集粪便样本和生活方式信息。下一代16S rDNA测序和系统聚类在两组粪便样本中没有时间趋势和个体内差异。在选定的39个优势属中，有24个属和23个属的季节和每日粪便样本存在显著的个体间差异(占人类肠道微生物区系总数的0.1%；占有率>；85%)。在季节稳定性和日稳定性方面，26属和35属的个体内和个体间的变异比达到了显著水平。发酵奶消费量的季节变化可能与双歧杆菌组成有关，但与乳杆菌无关。对于人类肠道微生物区系中的大多数优势属，个体间的变异明显大于个体内的变异。需要进一步的研究来确定人类肠道微生物区系对营养代谢、健康促进和疾病预防/发展的贡献。本文的在线版本(doi：10.1007/s00203-0151125-0)包含补充材料，授权用户可以使用。
Relationships between human gut microbiota, dietary habits, and health/diseases are the subject of epidemiological and clinical studies. However, the temporal stability and variability of the bacterial community in fecal samples remain unclear. In this study, middle-aged Japanese male and female volunteers (n = 5 each) without disease were recruited from the Sakura Diet Study. Fecal samples and lifestyle information were collected in every quarter and at each defecation for 7 continuous days. Next-generation sequencing of 16S rDNA and hierarchical clustering showed no time trend and intra-individual differences in both fecal sample sets. Significant inter-individual variations in seasonal and daily fecal sample sets were detected for 24 and 23 out of 39 selected dominant genera (>0.1 % of the total human gut microbiota; occupation rate >85 %), respectively. Intra- to inter-individual variance ratios in 26 and 35 genera were significantly <1.0 for seasonal and daily stabilities. Seasonal variation in fermented milk consumption might be associated with Bifidobacterium composition, but not with Lactobacillus. For most of the dominant genera in the human gut microbiota, inter-individual variations were significantly larger than intra-individual variations. Further studies are warranted to determine the contributions of human gut microbiota to nutritional metabolism, health promotion, and prevention/development of diseases. The online version of this article (doi:10.1007/s00203-015-1125-0) contains supplementary material, which is available to authorized users.